Food and Drug Administration (FDA) Disclosure:

The statements in this forum have not been evaluated by the Food and Drug Administration and are generated by non-professional writers. Any products described are not intended to diagnose, treat, cure, or prevent any disease.

Website Disclosure:

This forum contains general information about diet, health and nutrition. The information is not advice and is not a substitute for advice from a healthcare professional.

Cold Extraction Method for a THCA-CBDA Tincture

Discussion in 'Weed Edibles' started by madman1001, Mar 16, 2016.

  1. #1 madman1001, Mar 16, 2016
    Last edited: Mar 19, 2016
    Dissolving Acidic Cannabinoids for a THCA-CBDA Tincture

    Producing an Acidic Cannabinoid Solution has its challenges, primarily decarboxylation. Starting with fresh material is paramount; it’s mainly in Carboxylic acid form.

    Choosing an extraction method should be given careful consideration. Using a solvent like Ethanol as a carrier, some decarboxylation is possible if evaporated with heat.

    For ease and wanting to steer clear of activation we performed a dry ice extraction; eight ounces of fresh material with a 200 Micron Bag yielded 45 grams of glandular trichome’s-Keif.

    Liquid Coconut Oil is used as the carrier for the cannabinoids. It has a high fat content, is stable, pleasing to the palate and doesn’t solidify when refrigerated.

    A CAT X1000D Homogenizer Drive with a G-20 Knife Generator Shaft Assembly is used to dissolve the cannabinoids in Liquid Coconut Oil. The X1000D Homogenizer Drive is needed to provide the torque to process the high viscosity of the media; Liquid Coconut Oil & Keif.

    G-20 Knife Generator shafts are sealed to prevent abrasive material, like Keif from being processed through the shaft tube, thereby possibly damaging its O-rings, ball bearings, springs, and other components.

    The Knife Generator is engineered so that as powders are added into liquids they won’t congeal, it cuts while processing; splitting open the trichomes so the cannabinoids readily dissolve and diffuse in the carrier.

    For the experiment, we wanted to see what percentage of THCA- mg/ml is achievable with 5% by weight of Keif to 500ml of Liquid Coconut Oil, which comes to 25 grams.

    Heat is easily generated while processing due to the high viscosity of the media. To avoid the possibility of further decarboxylation, we chilled 500ml of Nature’s Way Liquid Coconut Oil to 38°F and placed it in a one liter beaker. 500ml is the minimum that can be processed in a one liter beaker with a G-20 Knife Generator, while introducing powders into liquids.

    The CAT X1000D is clamped on its drive stand so the Knife Generator is immersed in the Coconut Oil about a 1/16th of inch from the bottom of the vessel. The device is powered on with the speed set at 4000rpm’s. About 12 grams of Keif are added into the Coconut Oil. The motor speed is then increased to 8500 rpm’s and the vessel is moved around the shaft for about 45 seconds. The motor speed is decreased back to 4000 rpm’s and the rest of the Keif is placed into the oil. The motor speed is increased again to 8500 rpm’s for about 45 seconds while moving the vessel around the shaft. That completes the processing.

    We’re not quite sure if additional time processing; say a minute, would increase the THCA percentage. The temperature of the solution rose from 38°F to 75°F in 90 seconds, so we decided not to do any further processing with the X1000D Homogenizer. Instead, we placed the solution on the MCS78 Magnetic Hotplate Stirrer for an hour with no heat, just stirring. We don’t know if the hotplate stirrer is even needed. We thought it couldn’t hurt.

    We then vacuumed filtered the solution and sent a sample to the lab for a potency analysis. Here are the results: THCA Solution Analysis.

    This process is relatively fast, about 1 ½ hours from extraction to completed solution.

    Some additional testing will have to be done to discover if more time spent processing, two and a half minutes compared to 90 seconds increases the percentage of THCA. The additional time will require the vessel to be chilled to prevent further decarboxylation.

    homogenizer setup.jpg

    adding the keef.jpg
    keef added.jpg

    stirring the mix.jpg

    vaccuum and strainer.jpg

    final product.jpg

    Attached Files:

    • Like Like x 3
  2. So tell me --- why do you feel the need to blend the kief into the oil so well if you are going to filter it out later anyway?
  3. #3 OneE, Mar 18, 2016
    Last edited: Mar 18, 2016
    I would bet you could achieve that same level by just heating the oil to ~150-180f with minimal conversion. I can't imagine that the blades are 'cutting open' each and every trich - they are just bouncing off the blades at that point. Hard to justify such a spendy piece of equipment for such a simple process. From my knowledge of lab equipment, I'm guessing that unit goes $3-5k? Probably more since there looks to be lots of bells and whistles....

    I'd be more inclined to go with an ultrasonic horn homogenizer/tissue disruptor tuned for the trich size that would blast them apart, but that is even more expensive.
    • Like Like x 2
  4. Congrats on the successful extraction! What's the next step now that you've shown you can extract cannabinoids and retain acidic compounds by chilling the solution?
  5. All the fancy equipment is kind of overkill, isn't it? I can make a Green Dragon that's equally as inactive a whole lot easier than that. It's pretty simple, really. I'm glad it's helped your friend. The acids have uses where the cannabinoids, especially THC, can cause issues but to keep THCA from naturally converting over time, it should be refrigerated. Just a little tip for those with interest. :)
    • Like Like x 2
  6. #6 PsychedelicSam, Mar 24, 2016
    Last edited: Mar 24, 2016
    Here's mine. (I have more).

    I have slightly more THC than yours but that was the profile of the original bud and it is negligible in significance. However, the GD seems to have an overall better cannabinoid profile. Alcohol sensitivity is always something to keep in mind but so is allergies to coconut. The Green Dragon can also be used as a solvent transfer to oils and other mediums without significantly changing the profile.

    I'm not saying your process is flawed or anything. It's just that a grand majority of us here in this forum are not going to have access to the equipment that you are using so while it has been interesting to see, it's not really an option for most of us. :)
    • Like Like x 5
  7. Thanks for sharing @madman1001

    By liquid coconut oil are you referring to MCT oil?
    • Like Like x 1
  8. "The Green Dragon can also be used as a solvent transfer to oils and other mediums without significantly changing the profile."

    or can be concentrated in the transfer, if desired.

    Good to see you again Sam
    • Like Like x 4

Share This Page