As if we didn't have enough problems! Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production Viruses 2019, 11(9), 802; Lettuce Chlorosis Virus Disease: A New Threat to Cannabis Production Received: 5 August 2019 / Revised: 27 August 2019 / Accepted: 29 August 2019 / Published: 29 August 2019 (This article belongs to the Section Viruses of Plants, Fungi and Protozoa) View Full-Text Download PDF Abstract In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel, plants showed disease symptoms characteristic of nutrition deprivation. Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves. Next generation sequencing analysis of RNA extracted from symptomatic leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus that belongs to the Closteroviridae family. The complete viral genome sequence was obtained using RT-PCR and Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing. The two LCV RNA genome segments shared 85–99% nucleotide sequence identity with LCV isolates from GenBank database. The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted the disease from symptomatic cannabis plants to un-infected ‘healthy’ cannabis, Lactuca sativa, and Catharanthus roseus plants. Shoots from symptomatic cannabis plants, used for plant propagation, constituted a primary inoculum of the disease. To the best of our knowledge, this is the first report of cannabis plant disease caused by LCV. View Full-Text Keywords: Closteroviridae; Crinivirus; Bemisia tabaci; chlorosis; shoot propagation ▼ Show Figures Figure 1. Disease symptoms of lettuce chlorosis virus on old leaves of cannabis plants at the vegetative stage. (a) Yellowing leaves showing necrosis. (b) Purple leaves. (c) Chlorotic leaves. (d) Interveinal yellowing leaves showing necrosis, (e) Interveinal yellowing leaves. (f) Cannabis leaves of uninfected ‘healthy’ leaves.
just like battery hens get sick same rule applies to plants even humans keep them healthy and avoid gross batching imo! and never take you whitefly for granted