grafting for breeding?

Discussion in 'Advanced Growing Techniques' started by 1tokeovrtheline, Jan 30, 2010.

  1. I have heard all about the famous legend that you can graft hops and cannabis roots/stems. Of course this wouldn't produce thc in hops, but I wonder if you could use it for breeding plants to have better root systems? Of course the biggest question is whether or not this would carry on in the genetics of a clone or in seeds. I have heard that acapulco gold was made by using a technique where you cut the xylem and phloem (forget technical term for it sorry) in the last few days of flowering which cuts it off and makes everything rush to the remaining bud, sort of like cutting off your circulation making that area swell. If acapulco gold was bred this way, that would mean that they must have taken clones or seeds from the isolated portion and the genetics carried through right? I am wondering if the same could be applied to improve a strains root system by grafting.
     
  2. #2 RobertHope, Jan 30, 2010
    Last edited by a moderator: Jan 30, 2010
    Im sorry but i think theres no way thats how they got acapulco gold. acapulco gold is a strain from mexico and there is no way they went through all that trouble.
     
  3. lol well yea a lot of shitty weed comes from mexico, but this strain was developed in south america (columbia I think) and mexico, at its time it was one of the best strains in the world (impressive columbian strains came before shantibaba or neville or anyone, before white widow and northern lights and all that) plus the point of this really has nothing to do with acapulco gold, nor am I really sure that claim is true, which is why I said I have HEARD, its something called the preservation of truth, say what you know not what you think you know, and all I know is that I've heard that
     
  4. first of all, let me say that I know nothing about breeding marijuana, so take everything I say with a grain of salt.

    The "genetics" of any organism are just the representation of the DNA. Grafting two plants together is an environmental occurrence, not a genetic one. While the two parts may be able to survive together as one plant, the individual cells keep the DNA of their respective species. Basically, if you were to breed this plant you would either get the DNA of hops or the DNA of cannabis depending on which plant the reproductive organs came from, most likely cannabis.

    Doing this may change the way that this plant behaves, but its offspring will not be any different than if you bred the original marijuana plant before the grafting.

    Someone please correct me if I am wrong. :rolleyes::smoking:
     
  5. That is always what I thought too, but I have found more recently that environment can change dna, if this were not true then planting from seed produced from the same male and mother would basically be the same as cloning. DNA does change with environment, especially with mutations, but I am not sure whether this would be the case in grafting. I must admit my knowledge on this is not very in depth, and I may not understand it quite correctly, so really any feedback from someone who knows more about it would be appreciated.
     
  6. The example that you give doesn't really change the fact that the DNA from the hops will not get into the parts of the plant that is cannabis. This would be the equivalent of a white guy getting a kidney transplant from a black guy's kidney. If he were then to have kids they would not be black. If he got a testicle transplant (probably not possible, but I'm just trying to make a point) from a black guys testicles then he probably would have black kids because that is the reproductive organ from the black guy.

    What I'm getting at is that if the flowering part of the plant is the cannabis then it will only pass on cannabis genes and not hops genes, and the other way around.

    Hopefully I've explained this well enough... It makes sense in my head...:D:smoke:
     
  7. makes sense yea, but I know for a fact that graftiing is used for breeding some plants, and it does change the genetics, don't know if this is the case with weed though.
     
  8. I would like to talk about "tech terms", you will understand me better and I will you.
    You sounds very smart my bro.

    First, grafting is replacing stem or branch from two individual plants.
    Hence plant must be from same species its possible to made host for this botany experiment.

    Host means possession of rhizosphere where root system is included and at least one pair of health lamina for processing co2 and water (where is light included, naturally its cause termal source what is needed for cell kinetic energy and cause Photosyntesis II better to say for thylakoids) into carbohydrates for biosynthesis or respiration, whatever.
    Host lamina is very important in first couple days and together with root system will play big role to keep implemented part alive.
    Physical damage exist but transportation is still in function during xylem so upper part will get water to keep water pressure in the cells “at normal” and continue with photosynthesis.
    However, during cell signaling (what is connected with evolution) its possible to come to some changes into the nucleus from transcriptase proteins action.
    This can be different from specie to specie of course.
     
  9. oh thank god/buddha/shiva/tom cruise/oprah/the smurfs, someone who knows what they're talking about. Yea that's just what I am talking about, but I am wondering if anyone knows if this is true of cannabis sativa L., and since I asked about hops as well, if this would be true of Cannabaceae in general. I know these changes are possible for certain species, but I have never heard of it in cannabis, and of course that means that I have also never heard whether it would actually be effective enough to be worth the obvious stress it would cause. I was talking to another member about this as well and he came up with the crazy idea of grafting multiple strains in one mother for space restrictions. This sounds like an awesome fantasy to me, but as far as I know you can only graft a scion to a rootstock and not multiple scions to one rootstock or a scion to another scion. Because the xylem and phloem, etc are basically connected while grafting tape helps repair, it sounds possible to me, and I know from experience that broken stems can repair high up (supercroping gone wrong) so Ii would suppose that the hormones, etc would give the cells the ability to graft scion to scion as well as scion to rootstock. Of course if I am talking about this for breeding, then I must consider the possibility that keeping the mother for a while could eventually change the genetics between different strains within the plant. At any rate I have heard from people in the know such as Ed Rosenthal that cannabis does not graft easily, especially to hops, and so I would have to guess that multiple grafts on one plant would be difficult and very stressful, but at the same time perhaps over time you could actually breed a plant for this possible new development in growing. One thing I think would be hard for grafting hops and cannabis sativa, would be actually finding plants with the correct proportions to adequately transfer fluids, although I can't really say I know much about the actual differences between the two plants-as I have said, I know more aboot da ganj:smoke:.
     
  10. Obviously you do not read my post or you do not understand him ... not sure what is of this two.
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    Great, cause I don't know how xylem and phloem can directly repair broken or cutted stem ?
    What doing xylem and phloem to can repair stem ?

    Please tech terms, its easier for understanding.
     
  11. #11 lessismore, Feb 7, 2010
    Last edited by a moderator: Feb 7, 2010


    Acapulco Gold was and always will be a Mexican strain. It was discovered, grown, and distributed in Acapulco, Mexico in the 70's an early 80's. I have the version SSSC sold back in th 80's. No all Mexican is "shit weed". Michoacan, Oaxacan, and Acapulco Gold are all very good strains. The introduction of the Cartel controlled market there has changed many things in the genetics of true Mexican lines b/c they have introduced shorter flowering hybrids into the native strains for quicker an heavier harvests; withno regards in the quality of the offspring. Breeding is about selection; which most likely was not used in the major grow ops now ran by Mexican cartels. There are often many unsatisfactory results in breedin before true success is achieved. We must realize that the Cartel controlled market in Mexico sees money, not quality in the strains they produce and export to the illicit market.

    As for grafting. I am not sure how it would relate to breeding b/c the only benefit of grafting is to have several strains on one rooting system. Thi is very beneficial for MMJ states which have limits on how many plants can be grown. Breeding is about selection; the more plant one grows, the better stock you have to make your selections from. If you graft a strain to another strain, make it a mother, you will not have any selection, just a mother which will always be the same in clone form.

    I think grafting is a great way to keep seveal strains on one mother plant, but I do not see any use for the actual breedin process other than using well selected mothers and grafting them onto one plant. We also do not know how it will effect the strain. We know we can flower several strains from 1 plant using grafting, but how would it effect the strains over time if kept in mother form over a long period of time. We have seen where if a mother plant is stressed, it will alter the phenotype of the plant; ask AugustWest about some mothers which were stressed and the cuts all began to show very different characteristics.

    Grafting is an old process, however in the cannabis world it is still fairly new, and little is known about how it will effect the true characteristic of each individual strain used in the process.
     
  12. Well not to be a dick, but I'm not sure what "what doing xylem and phloem to can repair stem?" means. It sounds like english may not be your first language, and I don't mean to be a dick.

    If I understand correctly what you asked, here is what I meant: The cambium layer has the ability to grow back together if cut, and if hormones are applied then roots will grow out of it. This is what makes grafting and cloning possible, and for a successful graft of course this layer needs to properly connect and grow together. obviously you can't take a two month old scion and graft it onto a seedling rootstock or vice versa. On a much smaller scale I wonder if this may be part of what makes it so difficult to graft hops to cannabis- if it is difficult to get the cambium layers to line up properly, although I suspect it would be more the biological differences between the two cannabaceae that make it difficult for the plants to not reject each other-like a human or animal rejecting a transplant. I do have to admit that I'm a bit more like jorge cervantes- I've read a lot of what other people say, and my knowledge of more in depth botany, biotechnology, etc is by no means anywhere near a college degree (although I'm working on one!). If anyone has any more info on the specific biology of cannabis, or especially hops which I admit I know practically nothing about, it would be much appreciated.

    Also I want to say that a lot of people on here think I sound smart. I suppose that may be because I'm a novelist, writing is my life, and although I think I write very sloppily on here, it may sound like I'm trying to be smart. Please know that I'm not trying to be prideful or talk eloquently, and it has nothing to do with my knowledge of cannabis and biology in general. I'm just a regular guy who wants to learn more about this stuff, and I am working on what will hopefully be a doctorate in medicinal breeding. If I say anything incorrectly or anything I welcome correction, and I do not mean to assert that I am any sort of genius or have the experience of guys like lessismore.

    Also lessismore, I completely agree. The shitty weed coming form mexico-and other sources- seems to almost always be because of money minded efforts, which is unfortunate and just another reason why growing and breeding your own is so rewarding and often safer as well.

    Also grafting can be used for breeding in some plants, I'm just not sure that it is feasible or even biologically possible for cannabis. I do know that it is used in some crops such as soybeans however "The grafting technique appears to be an excellent method of inducing earlier flowering on late genotypes to permit their use in a crossing program. It is also a means of producing seed on genotypes that otherwise flower too late for seed production i~ a particular geographical area."
    Grafting as a Tool in Soybean Breeding -- Kiihl et al. 17 (1): 181 -- Crop Science

    and as OutdOorMaster said "However, during cell signaling (what is connected with evolution) its possible to come to some changes into the nucleus from transcriptase proteins action."

    Lessismore, have you successfully grafted multiple scions for your mother? I find that concept extremely interesting and useful because I too have serious space restraints.

    Out of the three of us I think its blatantly obvious I'm the least knowledgeable, so any tips or corrections from youse guys is tres appreciated.
     
  13. Grafting wouldn't carry over to offsprings.
     
  14. Man, I too have been reading and experimenting with this too.. (cannabis on cannabis) I guess the more i read though, it sounds like the grafted limb will stay the same as if it were on its original mother, and the rest of the plant will be the other strain? Am I getting this right?

    I wonder why/if/howcome if you do this, a cannabis on cannabis, say northern lights on bottom and acapulco gold on top, I would think it would change the golds Taste/smell/potency/ or something? but I guess not? This confuses me, I mean I can see why on one had they would stay the same, but what happens if after awhile it heals itself to like it never happend, you took a clone off it, would it just contain dna of gold, or both gold and lights??
     
  15. Um, no. That's like saying that all your kids will be identical clones so long as you have the same mother and father.

    It doesn't work like that, for plants or animals.
     
  16. no I'm saying that if it wasn't true that a plant takes its own genetics, then it would be more like cloning because all the kids would be identical, although not to either parent. But yea after I wrote that I realized it was a stupid thing to say, and a horrible way to say it, but I thought nobody would notice...but you did, so my bad I'll edit it
     

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