please share more about your experiences with tissue culture. I've read about this in mags but never seen anyone trying it themselves.
pictures, updates, progress...anything ya got. thanks
and I think we're all jealous of your growing space!
My experience has been a blast. It's great for the mad scientist type. My genetics have never felt safer. Caring for mothers is a bitch. Sure it's simple and easy but it requires energy from your wall socket and person. I look back at great genetics I lost for what ever reason as a newb and it was because of the caring for mothers around life. Thats why I got into TC; Not to mention the much needed vacation time. My genetics are secure and safe in an easy to manage system.
The pest and viruses & diseases (VD) that inevitably happen (given a long enough time line) stay with the cutting it's whole life. As time goes on desirable traits slowly but surely fad away as the plant combats environment, time and pest. The good news is the DNA is still the same and it can be revived and restored. Lost traits have been known to come back after a healthy or unhealthy clone is TC'd. Hard to germinate seeds or hard to clone plants become very easy to get propagated.
The virus and diseases that effect cannabis have a hard time keeping up with growth shoots. In cloning you need a decent amount of stem but the VD (viruses and diseases) are in the stem. This is why the clones on the street's are usually so crappy. By utilizing the meristem of the top fastest growing shoot you start with tissue free of VD. Proper sterilization allows the VD free meristem to propagate quickly under low light in a safe little glass home. Also unlike cuttings/clones you have options with TC. I can take that meristem and multiply it in a few weeks. In a 30 day period I could make hundreds of perfect replicas or make just one stem root; it's all in the hormones. Usually a plant is trading off auxins (positively influence cell enlargement, bud formation and root initiation) and cytokinins (influence cell division and shoot formation). In the TC environment you don't need roots to grow shoots. You provide the plant with everything it needs and the right naturally occurring plant hormones for the task you want it to do (rooting or multiplying).
Auxins can be introduced to grow roots for the many shoots you created. Cytokinins can be used to multiply tissue rapidly. You can graft plants together or work with a callus (generic tissue) to possibly make new strains from A &B stock without breeding and stabilizing. My favorite part is you can make a whole plant from just a piece of leaf if your Hormones are in correct proportions. Also, you can start with tissue from a plant in full flower.
Feel free to ask anything you like if you're left with questions. If people are interested I could do a step by step with pics of the whole process.
Edited by Hydro Druid, 10 February 2011 - 09:42 AM.