Thanks for the reply. Yeah these are a land race indica that would be a great addition to my breeding program. As far as storage goes, unfortunately I have no idea how they were stored. They were gifted to a friend still in the original sealed DNA genetics package. He in turn gifted them to me for some clones in the spring. For some reason he can't clone to save his life. I am gonna do as LD said and see what happens. I really hope it works out because this strain would be my only pure indica strain. Thanks for keeping your thumbs crossed, I have my toes crossed. Any how thanks for the encouragement and have a great day old man.
i know its old but did you get them to pop? i found a killer write up on how to make them go dormant, and bring them back, even if they haven't been stored right, as long as their is viable seed matieral inside this sounds like it would work. let me know, i am cutting and pasting it into a format usable here or that i can send to you if need be. aww, hell - it was written by a guest at another forum so i don't even have to give the forum any credit, let alone the person who wrote it. but I can't take credit for writing it only sharing and copy/pasting it. later!!
After reading this thread
and doing a good deal of fact finding/checking on seed germination (especially dormant, old seeds) I think I've got something worth posting...I hope everyone will lean something, I know I did!
Dormant Seed Germination 101
Part 1: Intro
My main reason for posting is to assist others (and myself!) in germinating old, dormant cannabis seeds. Not only do I hope to generate discussion, but I hope this can become a "germination reference manual" if you will. The info in this thread is relevant to all cannabis seeds and germinations.
I am concentrating on the causes of seed dormancy, associated germination problems and their solutions
. I use the same techniques to germinate old and young seeds alike...but for the sake of this thread's clarity and simplicity I'm concentrating on old, dormant seeds. You should be able to germinate viable
older seeds (20+ years) with the information and guide herein.Main causes of germination problems:
a.) Seed Viability:
If the embryo is alive the seed is viable, if the embryo is dead the seed is not viable.
A seeds viability is affected by a number of different conditions. Some plants do not produce seeds that have functional complete embryos or the seed may have no embryo at all, often called "empty seeds". Pathogens can damage or kill the seed while it is still in the flower or after it is harvested. Environmental conditions like improper long-term storage and the age of the seed affects its health and germination ability. Seeds containe a living embryo and over time cells die...they cannot be replaced...sometimes seeds are just to old.
b.) Seed Dormancy:
There are two main groups (internal and external) of seed dormancy which contain five possible causes of the dormancy. This section is very important because it helps us define the type
of dormancy so we may then better understand how to break the dormancy and germinate the seed. Seeds may become dormant in a few weeks or even a few days under extreme conditions.
("innate dormancy" - internal)
Is when a seed fails to germinate under optimal environmental conditions. This is caused by conditions within the seed that prevent germination under normally ideal conditions.
("endogenous dormancy" - internal)
Is caused by conditions within the embryo itself. This can be caused when seeds have not gone through "stratification" (to simulate "after-ripening"). This can prevent seed germination until the chemical inhibitors are broken down or are no longer produced by the seed after stratification.
("double dormancy" - internal/external)
Is when a seed has more than one type of dormancy...this is fairly common.
("exogenous dormancy" - external)
Is caused by conditions outside of the embryo. For example, if the shell is too hard or thick it can be impermeable to water or the exchange of gases...the seed coat physically prevents water from reaching and activating the embryo.
("seed quiescence" - external)
Naturally occurs when the seed dries after being harvested (aka "seed hibernation"). We are not concerned with seed quiescence because this is not a problematic dormancy as the seed will germinate when the environmental conditions are optimal.
c.) Seed Hibernation:
Is when seeds fail to germinate due to a lack of environmental factor(s) essential to life (water, sunlight, nutrients, etc.). Hibernation is generally a short lived experience as it morphs into innate dormancy after a few months to a year or more. We are not concerned with hibernation because the seed will germinate if given the proper environment.
-----------------------------up next...solutions for different types of dormancy...then the step-by-step guide!
01-10-2008, 08:45 PM
Dormant Seed Germination 101
Part 2: "Breaking dormancy"
In this section I will detail the methods used to "break" (bring out of dormancy by germinating) seeds.Types of dormancy and ways to Break the Dormancy:
The methods I will describe are horticultural standards but I have trailered them for cannabis seeds and their natural "after-ripening" process (e.g. rough handling [animal stomach, etc], winter of 2-6 months [depending on genotype], etc).
True dormancy ("innate dormancy" - internal)solution:
Cold Stratification ("moist-chilling")
This method is meant to simulate the winter a seed would naturally encounter in the wild. Seeds in the temperate zones of the world often remain covered with humus for weeks or months and are subjected to chilling temperatures which help break the rest period.
Seeds are placed in a lightly moistened bunch of organic matter (coco-coir) and placed into refrigerator.
One thing to consider is the seeds genotype; is it mainly stative, indica, a landrace? This question is important and should be used to customize cold stratification to mimic the seeds natural after-ripening environment. A haze should be kept at warmer temps (40-45F) for a shorter time period (1-2 months)...where an Afghani should be kept at cooler temps (35-40F) for a longer time period (2-3) months. What about moisture content? Is the organic matter (hummus, dung, etc) that the seeds naturally after-ripen in moist or dry?
Benefits of cold stratification:
-softens shell to facilitate h20 absorption during "seed priming"
-seeds imbibe water and in the moist chill they after-ripen
Physiological dormancy ("endogenous dormancy" - internal)solution:
Physical dormancy ("exogenous dormancy" - external)solution:
The seed will not germinate until the seed coat is altered physically because the seed shell is hard and moisture cannot penetrate to the embryo. Any process of breaking, scratching, or mechanically altering the seed coat to make it more permeable to water and gases is known as scarification.
In nature, scarification often occurs by falling seeds, freezing temperatures or microbial activities. These modify the seed coat during the winter. Scarification can also occur as seeds pass through the digestive tract of various animals.
Benefits of scarification:
-scratching shell allows greater h2o penetration from outside the shell
-trimming off very tip of seeds allows h2o to enter, activate embryo and begin softening shell from inside
Induced dormancy ("seed quiescence" - external)solution:
Proper germinating environment
IMO this is the same as "seed hibernation", both can be solved by giving seeds the proper environment...see my next post for that!
Combinational dormancy ("double dormancy" - internal/external)solution:
See all 'solutions' above...
up next...the guide...then the final chapter...why I'm using what I'm using in the seed soak
01-10-2008, 09:08 PM
Dormant Seed Germination 101
Part 3: The guide
1. wash hands in good medical grade soap and sterilize all tools. I want to wear gloves but it's damn hard to hold the seeds
2. throughly scarify seeds w/mild sandpaper. You can put seeds into a film canister with sandpaper glue to it; then just shake the canister to scarify the seeds.
3. boil a quart of distilled h2o for 10 minutes
4. put pre-expanded and leeched hand full of coco coir into boiling water and let boil for 5-10 min. Take coir out of h2o can let cool and dry out until very lightly moist (shake and squeeze).
5. put seeds in small bunch of very lightly moist coir, very lightly packed into a cup. Put the cup into fridge for 2-3 months at 40F to simulate the soil in the winter. Make sure to check seeds often (daily) to make sure they are not germinating in the coir and it's not too cold. (remember to think about your seeds genotype!)
6. use sterilized
razor to cut off the very tip of the pointy side of the seed (the side that opens, not the hinge!)...careful not to crush/damage shell or seedling inside! (thanks c-ray, good one!)
7. soak seeds (aka "on-farm seed priming") in with the following ingredients and keep in a dark place. Use aquarium heater to heat water to 80F and soak for about 20 hours. (I used cc not ml as it's way easier to measure small amounts of liquids with a syringe)
1 liter distilled h2o kept at 80F degrees
.5cc homemade AEM (1:10,000=.5cc or .5ml per liter) <--not necessary but good to have
1cc blk strap molasses
1cc earthworm castings tea
2cc seaweed/kelp liquid extract
2cc fulvic acid
1 drop superthrive
ph of 6.5
8. put humidity dome over seeding mat and set the mat to 80 degrees. To build up humidity and sanitize the dome lightly spray inside of dome with mix of 1 liter h2o and 2cc 3% h2o2
9. put seeds on new, clean paper towel and fill spray bottle with seed soaking solution (e.castings, superthirve, etc...not
AEM). Spray the seeds and the paper towel to moisten both of them. Then lightly fold the p.towel over seeds to keep seeds moist.
10. put p.towel and seeds into humidity dome.
11. check and open p.towel a few times a day to ensure fresh air and proper moister content. seeds can take 2+ weeks to crack the shell.
12. if seeds begin sprout then use tweezers, razor blade, etc to split shell to allow seedling to easily sprout. Put back into p.towel and allow seeds to germinate further...until sprouts root is half the length of your pinky fingernail.
13. transplant germinated seedlings into medium of choice.
------------------------------up next...why I'm using what I'm using in the seed soak
01-10-2008, 11:57 PM
Dormant Seed Germination 101
Part 4: Seed priming mix explained
Wanna know why I suggested each ingredient for the seed soak? well here ya' go...
Steam distilled h2o
This is the purest water you can buy it's pretty inexpensive too.
Earthworm castings tea
Contains a good deal of beneficial microbes (if fresh), along with macro nutrients, humics and
they should have a positive symbiotic interaction with the seeds.
This contains a good deal of natural hormones, minerals, auxins, cytokinins and gibberellins along with P and K which is good for seed germination.
Activated Effective Microorganisms (not necessary but good to have)
I'm using AEM as a form of stratification, they should weaken shell and
have a positive symbiotic interaction with the seeds.
Black strap molasses (organic)
Used as a food source for the AEM and it adds natural sugars to h20 which has been found to reduce transplant shock.
If seeds are soaked with fulvic then they produce stronger and more vigorous roots, earlier too.
This contains B vitamins which reduces shock and contains triacontanol which promotes root growth.
OK, well that's it...for now! any thoughts, questions, corrections?
Edited by lbezphil2005, 31 May 2013 - 07:55 AM.