*How Too* Pythium Root Rot Treating

I've been away for sometime and figured it was time to up date this thread. Below is a ton of info from myself and others. If you want the tech info then read all the info below. Also I would like to "Thank All the kind comments i have gotten from users concerning this thread. No, I haven't read threw the thread, the comments i am referring to are the PM's and emails as well as rep. Also, I welcome any users add-ons in the form of instructions or helpful hints to others. But I won't debate the info here or engage in a discussion of what is better than that and some info may be wrong. If you feel the info here is wrong then please post >Why< then >What works< then >document<, your findings so others can research as well.

Since i started this thread I've tried many others so called fixes/treatments and some worked some didn't so what your about to read is a quick fix Tut.


Before I begin know now that if your going to fight this it needs to be understood that it is a long battle. If you need your grows harvested on time then fighting root rot is not the smart choice. If you have the rot and want it gone NOW the only way is to kill everything and then replace everything NEW. Yea, it is ruff but it is the only way to be sure. Once a plant is infected or a system then it will always carry the bug in some form. The problem is no system or custom built system that I know of lends itself to complete sterilization because parts are rubber, plastic, and so on. To serialize the right way parts would need to be sterling steel so they wouldn't rust and be able to with stand heat upwards or 250F. To my knowledge no system I have seen is constructed like this. So if you have say 4 plants in independent systems IE Bubble Buckets or you can place the plants in there own buckets then you can choose to fight but if you have a 10 bucket system hooked in series with a pump or drippers then you’re fighting as losing battle in terms of TIME, MONEY. But no one is saying you can't try. The sooner you find the rot the better, when early it is very easy to kill but after 72hrs complete death becomes much harder.


Next let’s get something outta the way right here, H2O2 don't work in any manner or form, next Rockwool is a REALLY bad idea in any form because it can not be cleaned and the plants can't be transplanted. Rockwool does have it's place in growing but in a full blown hydro system like Bubble buckets or High Flow DWC in my view it shouldn't be used. Ebb and grow or flood and drain yes Rockwool is a god send but not in DWC. If you choose to use these items then fighting isn't a good idea. Yes I know I am writing "fighting words" concerning Rockwool but again, I didn't say it should never be used, just not in DWC and if you have had the Rot just one time then it should never be used.
If you are a Hydro Grower you will get the Rot at some point. Again, I know there are grows of 10yrs plus that have never had it and no I have no idea why but every professional even the ones a Epcot have all told me Rot is a fact of life so I'm repeating what I've been told.
Treatment
Tools you need to treat 1 Plant.


1.\tClean new black bucket.
2.\tOffbrand bleach or commercial med grade bleach. Name brand is fine but not needed
3.\tStore bought peroxide.
4.\tAlcohol as pure as you can find. Even 80 proof vodka will work.
5.\tBrush
6.\tHydrotron cleaned and sterilized. After cleaning (to sterilize) let rocks soak in a 50/50 mix of peroxide and water for 15min. Rinse clean.
7.\tRemove the filter from any air pumps, on bottom or buy new along with air lines and stones, drippers, etc.


If 4x4 rockwool, mix one cup peroxide in Gal water then pour into a bowl and place plant and cube into the water. If any algae make sure solution bubbles it away leave for five min. then rinse in plain water. Then back into solution one more time followed by water. Cut away as much of the cube sides as you safely can. Inspect roots, any brown (this is important if your nutes stain your roots then using brown to find problem roots isn't going to work. Do the best you can, I myself stopped using any additive that stained the roots or turned the water brown long ago that includes organics) roots cut away also make sure to keep the tool used for cutting away from the grow room from now on. I keep a rot cutter in the garage. Remove as much as you personally feel safe doing. No set amount here as you will see later on. Run the roots under running cold tap water to rinse any stray leftovers away.

Fill bucket with a 500ppm nutes solution and PH to 5.5 no lower than 5.0 no higher than 5.5. Add new lines, stones, and pumps or pump with removed filter. If you cut away allot of your rock cube refill with hydrotron cleaned as above or loose rockwool. Then re-add the plant to the system. Also, any meters you used on the infected plants, nute cups, bottles, floor covering, wall covering will all need to be cleaned.

Clean meters per manufacture instructions clean the tools and walls with a 9 parts water to 1 part bleach mix and rinse with clean cold water. More bleach isn't better 9/1 is all that is needed. If you can hydro stores sell root rot foggers for rooms get one and use. That's it your done. The plant will start with the older leafs 1st getting food from them, as they die off remove when 20% of the green is all that is left. Plant may sag or look sick, because it is, just leave it be. No PH adjust either, again leave it be, that's what the 5.5 is for. Inspect daily, if you see any brown in water or foam on the top of water pull the plant and do again from scratch. Do again in 3 days and keep doing every 3 days cutting away brow roots or slimly roots till you see new roots forming. No organics of any kind during this period, also make sure the new roots can get water. Do this by judging how far the bubbles are traveling into the root zone (if top feed or drippers skip this step).

New roots will form in new areas. So they may be well above the old one's. You can also pour a 1/4 cup of 500ppm nutes down the top if you really worried. No temps above 75f for any reason. You don't need fancy water either, normal tap is fine.


A word about AIR, In hydro the rot loves air and needs it. without it rot can't grow. Most of the rot info on the net is about dirt and hydro growers makes the mistake of applying the same rules to hydro. The first place rot often shows in on your air stones. To test this I put 2 stones in a bucket with an infected plant and filled the water to the bottom of the pot. Waited 3 days and sure enough, no rot on the roots but both stones were covered and eating all the nutes. In effect the stones drew the rot off the roots and to them. NO DO NOT TRY THIS AT HOME. Repeated the same test but this time with one stone. This time some roots had slime but the stone had more. Next, a bucket with rot and added a plant with no rot, 2 stones, filled to pot6 bottom. 3 days, no rot on roots, only the 2 stones. removed plant and it grew to harvest.

Then at a convention I happened to be telling this story to a FF rep and a guy from Advanced heard me. He informed me that the white snot root rot needed air to grow and to only ever use 1 stone per bucket and max 2 in the normal 5gal res. He also said I managed to figure out what his company spent years researching. To much air in hydro isn't always a good thing which is contrary to what most of us are taught online and in local hydro shops everywhere.

No, I'm not going to argue this point, to prove me wrong test for yourself then post results.


To clean a 10 bucket system that can't be replaced. Drip lines or DWC. Warning not for environmental aware growers. Find a med product called Cidex on eBay. Get two gallons, no the shit the hydro shop owners sell isn't the same. Clean the system as above, then mix Cidex per instructions and run per instructions. Drain, then overfill the system 3 times and drain each time. Then fan dry system. Cidex isn't cheap but it will kill all forms of any micro nasty known to man on plastic or metal, sorry you'll need new rubber. If hoses are a form of PVC they can work with Cidex. But this should only be done when the plants are cured. Again, No INFECTED plants.

Always cut off affected roots- try as best you can to stay away from anything brown in a hydro system if you are fighting rot or until flower when the roots are growing well- use clean hydrotron that way if there is a problem transplant is a breeze- the new white high dollar grow cubes are fine to use as long as no problems arise- you can use a pressure cooker to sterilize CLEAN hydrotron at 270f at 15psi for 30min tattoo section of ebay has stovetop units cheap- water temps 98/75 no more- room temps never over 78- hepa filter outside air into grow room- do a little reading on root pruning it works the same for hydro


Many growers run into the dreaded root rot. Many already know how to treat it but many new growers don't. So this thread is for the noobs.

I myself have been having much trouble with the Pythium rot. So this thread will have some info that I found. Right now my plants are covered with white snot and I've been fighting it for months. Many growers feel that if your plants get root rot it many times is just better to kill the grow and start over. I myself try to fight it. I hope the info below will help some of you or at least get you going in the right direction.

If any other members have any advise please feel free to post. I added tags to this thread so it can be found in future searches.

The etiology and epidemiology of Pythium root rot in hydroponically-grown crops are reviewed with emphasis on knowledge and concepts considered important for managing the disease in commercial greenhouses. Pythium root rot continually threatens the productivity of numerous kinds of crops in hydroponic systems around the world including cucumber, tomato, sweet pepper, spinach, lettuce, nasturtium, arugula, rose, and chrysanthemum. Principal causal agents include Pythium aphanidermatum, Pythium dissotocum, members of Pythium group F, and Pythium ultimum var. ultimum. Perspectives are given of sources of initial inoculum of Pythium spp. in hydroponic systems, of infection and colonization of roots by the pathogens, symptom development and inoculum production in host roots, and inoculum dispersal in nutrient solutions. Recent findings that a specific elicitor produced by P. aphanidermatum may trigger necrosis (browning) of the roots and the transition from biotrophic to necrotrophic infection are considered. Effects on root rot epidemics of host factors (disease susceptibility, phenological growth stage, root exudates and phenolic substances), the root environment (rooting media, concentrations of dissolved oxygen and phenolic substances in the nutrient solution, microbial communities and temperature) and human interferences (cropping practices and control measures) are reviewed. Recent findings on predisposition of roots to Pythium attack by environmental stress factors are highlighted. The commonly minor impact on epidemics of measures to disinfest nutrient solution as it recirculates outside the crop is contrasted with the impact of treatments that suppress Pythium in the roots and root zone of the crop. New discoveries that infection of roots by P. aphanidermatum markedly slows the increase in leaf area and whole-plant carbon gain without significant effect on the efficiency of photosynthesis per unit area of leaf are noted. The platform of knowledge and understanding of the etiology and epidemiology of root rot, and its effects on the physiology of the whole plant, are discussed in relation to new research directions and development of better practices to manage the disease in hydroponic crops. Focus is on methods and technologies for tracking Pythium and root rot, and on developing, integrating, and optimizing treatments to suppress the pathogen in the root zone and progress of root rot.
Pythium root rot is ubiquitous and frequently destructive in almost all kinds of plants produced in hydroponic systems, including cucumber, tomato, sweet pepper, spinach, lettuce, arugula, and roses. In Canada, root rot epidemics continually threaten the productivity of greenhouse vegetables and flowers such as chrysanthemums in hydroponic troughs on mobile benches which, compared to ground beds, would have allowed substantial increases in production efficiencies. Pythium root rot is also considered a potential threat to plant biomass production in manned space vehicles and at extraterrestrial installations in projected space missions (41, 70, 92). Indeed Pythium has already been encountered on plant materials in space vehicles operated in earth orbit. In greenhouse and growth room studies, Pythium root rot became severe in hydroponic tobacco, Arabidopsis and antirrhinum, which are key plants employed for genetic, molecular and physiological studies (N. Ortiz, W. Liu, & J.C. Sutton, 2003 unpublished observations). Thus, the problem of Pythium root rot needs to be resolved in diverse commercial and research situations.

Management of Pythium root rot in the production of hydroponic crops is generally a difficult challenge. Extraordinary sanitation measures do not necessarily exclude or destroy the causal pathogens, and once initiated, epidemics are difficult to contain. Recent advances in knowledge and understanding of the etiology and epidemiology of root rot, and in methods and approaches to control the disease, are providing a framework for major improvements in root rot management and in the overall health and productivity of hydroponic crops. This review considers recent findings in small-scale and commercial hydroponic systems in relation to methods and technologies to optimize root rot management.

Hydroponic systems and the root environment

In cool temperate climates such as in southern Canada, hydroponic crops normally are grown in greenhouses with sophisticated systems for controlling conditions of the microclimate (temperature, humidity, carbon dioxide, light) and nutrient solution composition (pH, and dissolved oxygen concentration). On the other hand, in warm or tropical climates such as in São Paulo and Belém, Brazil, hydroponic crops are grown without sophisticated climate control, but with at least partial protection against harsh weather conditions, and with standardized nutrient solution. In such climates, hydroponic crops generally are produced in greenhouses constructed with wooden or concrete frames and covered on top with clear plastic film to exclude rain, and, in some instances, with screening against intense sunlight. Wooden lattice or plastic film may be used on one or more sides for protection against wind and rain.

Roots of hydroponic plants either grow in the nutrient solution only, or in rockwool (stone wool), coconut fiber, sawdust, sand or other medium that is irrigated with nutrient solution. No soil is employed. Some crops, especially flowers and other ornamentals, are grown in single- or multi-plant containers with a rooting medium that is irrigated via tubing from above or by means of a trough below the container. Hydroponic vegetables often are grown in slabs or blocks of rockwool, coconut fiber or other medium enclosed in plastic film and fed with nutrient solution via plastic capillary tubes (Fig. 1). Others are grown in troughs formed from black-on-white plastic on the greenhouse floor, or in troughs of rigid plastic positioned at ground level or on benches. Nutrient solution is circulated through the troughs, which may or may not contain a rooting medium. Troughs usually are arranged in parallel and interconnected into large systems that accommodate thousands or tens of thousands of plants (Fig. 2). Lettuce, arugula (Eruca sativa Mill. or Eruca versicaria subsp. sativa Shallot), Nasturtium officinalis R.BR., and other small-sized plants are sometimes grown on gently sloping sheets of corrugated plastic or other material, and nutrient solution is allowed to flow down channels in the sheets and through the root zone of the plants. Nutrient solution in the various systems is either circulated once or is continuously recirculated. In Canada, the hydroponics crops industry is in transition from continuous or frequent discharge of used nutrient solutions into the environment to continuous recirculation through root zones of crops. Hydroponic systems are being adapted for continuous recirculation over concerns about pollution of water resources with greenhouse effluents and to conform with new environmental legislation.


The main species of Pythium reported to cause root rot in hydroponic crops are P. aphanidermatum (Edson) Fitzp., P. dissotocum Drechsler, P. ultimum Trow var. ultimum, and members of Pythium group F (4, 14, 23, 28, 39, 61, 68, 73, 82, 86, 106). Pythium aphanidermatum, P. dissotocum, and Pythium F produce abundant zoospores, while P. ultimum var. ultimum produces zoospores only rarely. Zoospores are produced asexually in sporangia and associated vesicles. The sporangia of P. aphanidermatum are filamentous and lobed (or inflated), while those of P. dissotocum are filamentous, dendroidly branched and not inflated or slightly inflated (112). Pythium group F is characterized by filamentous non-inflated sporangia (82, 100). Each of the species is able to produce oospores (thick-walled sexual spores) in infected roots and in the rhizosphere. These Pythium spp. each attack a wide range of host plants. A few other species, such as P. intermedium and P. irregulare (82, 101), have also been reported in hydroponically-grown plants. The genus Pythium belongs in the family Pythiaceae of the class Oomycetes, members of which were regarded as fungi for over a century and a half. However, Oomycetes are now commonly described as fungal-like and assigned to the Kingdom Straminipila (16).

Sources of pythium inoculum in hydroponic crops

The principal species of Pythium that attack hydroponic crops frequently occur in soils and plant residues in greenhouses and outdoors (61, 73, 86). Inoculum of Pythium spp. can be introduced into greenhouses and hydroponic systems in many ways including airborne dust, in soil and plant fragments on greenhouse tools and equipment, on people's footwear, and in water used to prepare nutrient solution (39, 59, 75, 78, 95, 99). Pythium spp. were reported also in peat brought into greenhouses for use in rooting media (23, 87).

In Canada, transplants are a common source of Pythium in hydroponic vegetable crops. Some hydroponic vegetable growers produce their own transplants, but a majority obtain them from specialist producers who supply the plants in rockwool cubes. Growers who produce their own transplants often do so on benches in greenhouses that are not otherwise used for crop production. In several instances P. aphanidermatum and P. dissotocum were easily recovered from soil trapped in benches used for growing transplants, and the pathogens were frequent in roots of cucumber, pepper, and tomato transplants (W. Liu & J.C. Sutton, 2000-2003, unpublished). Specialists generally begin production of transplants in rockwool plugs (about 2.5 x 2.5 x 4.0 cm) contained in plastic trays, and subsequently transfer them to rockwool cubes (about 10.0 x 10.0 x 6.5 cm) positioned in rows on laser-levelled concrete floors. Every few hours the floors are flooded with nutrient solution and allowed to drain so as to keep the cubes moistened. In this type of ebb and flood system, the nutrient solution is pumped from tanks below ground level, through pipes beneath the floors, and out onto the floors before draining back into the tanks. Despite extraordinary sanitation measures, some plants produced in these systems were found to be infected with P. aphanidermatum and P. dissotocum (Sutton, J.C. & W. Liu, 2002, unpublished). The presumed inoculum source was the underground plumbing, which possibly harbored oospores and mycelium. In many instances, infected transplants from various production systems were symptomless when ready for shipping to growers.

It is possible that transplants of hydroponic salad crops such as lettuce, arugula, and Nasturtium officinalis also become infected by Pythium prior to being set out in hydroponic systems. In Pará State, Brazil, transplants are often produced in cells of seedling flats containing various rooting media, and positioned on benches sheltered from rain, wind, and direct sunlight. Some growers utilize styrofoam trays floating on water in tanks. Infection of transplants by Pythium seems possible in these systems, however we are not aware of any reports to confirm or refute this.

Hydroponic materials used previously for crop production may frequently harbor Pythium. Some growers re-use rooting media such as slabs of rockwool or coconut fiber, without sterilization or other measures to destroy pathogens in the media. This practice allows carry-over of inoculum of various pathogens, including Pythium spp., to the subsequent crop. Pipes, tubing, tanks and other plumbing components of hydroponic systems are often potential sources of Pythium in successive crops, even when the systems are treated with disinfectants. In studies in small-scale hydroponic systems, the chemical sterilants Virkon (potassium monopersulfate; Pace Chemicals, Burnaby, Canada) and Chemprocide (didecyldimethyl ammonium chloride; Dispar Inc., Joliette, Canada) were only partially effective in destroying P. aphanidermatum (C.R. Sopher and J.C. Sutton, 2003, unpublished). Sodium hypochlorite was completely effective, but can corrode some components of hydroponic systems. Oospores of Pythium spp. can frequently be found in biofilms, mucilaginous materials and root tissue fragments in hydroponic plumbing (C.R. Sopher and J.C. Sutton, 2003, unpublished) and are undoubtedly a principal form of inoculum. Oospores are also resistant to chemical sterilants. Oospore populations are also known to survive for months or years in field soils (2, 61, 108).

Insect vectors are considered important means by which Pythium and other pathogens are introduced and dispersed in hydroponic crops. In North America, fungus gnats (Bradysia spp.) and shore flies (Scatella stagnalis Fallen.) were reported to acquire Pythium by external contamination or ingestion (27, 29, 40). Pythium oospores were found in the digestive tracts of larvae and adults of each of these insects. In fungus gnats, oospores acquired during the larval stage remain in the digestive tract during pupation, and can be aerially transmitted generally in a viable state by the adults and eventually excreted in the frass (40). In Canada, fungus gnats are found wherever greenhouse crops are grown, and readily enter greenhouses through doors and ventilators (37). Their larvae feed on fungal mycelia and organic residues in soils, soilless mixes, hydroponic media, and nutrient solutions. The larvae are also known to feed on roots and root hairs of cucumbers and other plants, thereby making wounds through which pathogens may invade. It is likely that similar or different insects are factors in epidemics of root rot in hydroponic crops produced in warm temperate and tropical climates, but we have not encountered any reports on this possibility.

Infection of roots by Pythium

Pythium aphanidermatum, P. dissotocum, and Pythium group F infect roots of hydroponic plants by means of zoospores and mycelia (21, 73, 82, 86, 99, 122). The initial (primary) inoculum in root rot epidemics (that is, the inoculum which initiates the epidemics) is chiefly zoospores produced from sporangia formed by germinating oospores, or perhaps by mycelium (31), in plant residues, soil, hydroponic pipes and tubing, and other inoculum sources in the crop environment. Generations of zoospores arising from sporangia formed on infected roots of the hydroponic crop are a principal form of subsequent (or secondary) inoculum in root rot epidemics. Mycelia are the principal units of inoculum of P. ultimum var. ultimum, which produces few or no zoospores and is better adapted to conditions of greenhouse soils and soilless mixes than to systems with circulating nutrient solution (43). The reader is referred to Martin & Loper (61) for details of P. ultimum var. ultimum.

Consistent with general acceptance by plant disease epidemiologists, the term infection is used here to refer to the process of establishment of a parasitic relationship of the pathogen in the host (9). In Pythium zoospores this process includes zoospore encystment at the root surface, synthesis of a thick cell wall, adhesion to the root surface, germination and germ tube growth, penetration of the root surface, and sufficient post-penetration development to allow the newly-formed colony to function independently of the germinated spore (33). Zoospores of P. aphanidermatum and other Pythium spp. were reported to penetrate non-wounded surfaces of all portions of young roots, including root cap cells, root hairs, and regions of meristematic activity, cell elongation, and cell maturation (21, 48, 56, 118). In general, however, root tips, elongation zones, and young root hairs are frequently penetrated. Pythium aphanidermatum and other Pythium spp. have been reported to penetrate roots directly by means of penetration pegs, fine hyphae, and enzymatic action (21, 28). Formal descriptions of P. aphanidermatum and P. dissotocum include appressoria (112), and indeed some authors observed appressoria or appressoria-like structures on roots by means of transmission or scanning electron microscopy (15, 21, 86). In other studies, however, scanning electron microscopy did not reveal appressoria (28) or appressoria were not mentioned (21). Production of appressoria is known to vary with temperature and pH (21), and it is possible that incidence and density of appressoria during epidemics of Pythium root rot in hydroponic crops is highly variable. Besides direct penetration of roots, P. aphanidermatum and other Pythium spp. are able to infect wounded tissues, such as sites of emergence of lateral roots and sites of attack by larvae of fungus gnats.

Findings in several studies indicate that root mucilage on surfaces of roots and in surrounding nutrient solution is an important factor influencing infection of roots by Pythium hyphae, and in promoting Pythium populations in the root zone. Pythium group F, P. ultimum var. ultimum, and other Pythium spp. penetrate roots in zones of increased mucilage production such as junctions of cortical cells, zones of elongation, and at the base of lateral roots and root hairs (43, 67, 86, 118). Cucumber plants often produce abundant root mucilage that accumulates in the nutrient solution. Zheng et al. (122) reported on the role of this mucilage in epidemics of root rot caused by P. aphanidermatum. They found that the pathogen was more frequent in roots with associated mucilage than in those lacking mucilage, and that the amounts of mucilage correlated positively with root browning. These findings, combined with microscopic observations of Pythium hyphae in mucilage and roots, indicated that the mucilage supported prolific growth of P. aphanidermatum and served as a food base from which hyphae of the pathogen were able to invade the roots, including old roots which zoospores normally do not infect.

Colonization and symptom development

Colonization of plants by P. aphanidermatum and P. dissotocum in hydroponic plants is normally biotrophic in initial stages and subsequently necrotrophic (73). In the biotrophic phase the roots are colonized without development of overt symptoms, a condition sometimes referred to as subclinical (98). In the necrotrophic phase, the roots become discolored, generally as a hue of brown, grey-brown, reddish-brown, yellow-brown, or yellow, depending on the type of host and species or isolate of the pathogen. Pythium group F was found to colonize roots of hydroponic tomato plants without inducing visible symptoms under optimal conditions for plant growth, but some strains caused severe necrosis especially in stressed plants (14, 85, 86).

Root colonization by P. aphanidermatum, P. dissotocum, and Pythium group F is both intercellular and intracellular (21, 71, 85, 98). Haustoria-like structures were reported in cells of spinach and pepper roots infected by P. dissotocum (71, 98). P. aphanidermatum normally colonizes the cortex of pepper roots (73), while P. dissotocum has been found in the stele of immature root tips of strawberry (69) and tends to colonize epidermal cells of roots (21). Each of these pathogens increased cytoplasmic granulation in cortical tissues of pepper (73). Pythium group F was found to colonize the epidermis and outer cortex of tomato roots, inducing marked disorganization of the host cells in a phase of pathogenesis interpreted as necrotrophy, and to subsequently ingress to the inner cortex and stele, where it induced various kinds of host defense reactions (85).

The necrotrophic phase of Pythium root rot in hydroponic pepper plants is marked by root tip browning and expansive browning (P. aphanidermatum) or yellowing (P. dissotocum) of the roots (71, 73). All isolates of P. aphanidermatum and P. dissotocum also produced architectural changes in the root systems, chiefly stunting, stubbiness, and root proliferation (73). Some isolates of P. dissotocum produced swelling of roots and proliferation of callus cells. Similar kinds of symptoms were found in hydroponically-grown chrysanthemums (58), lettuce, antirrhinums, (J.C. Sutton, W. Liu, M. Johnstone, and N. Ortiz-Uribe, 2002-2003, unpublished observations). In all hosts, roots were colonized by the Pythium isolates well in advance of expansive root browning or yellowing, such that much more root was colonized than exhibited overt symptoms as illustrated for chrysanthemum (Fig. 3). Thus, in agreement with comments of Kamoun et al. (52), root necrosis did not represent a hypersensitive reaction or incompatible response in which the host cells are quickly killed and advance of the pathogen is blocked.

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Root browning is a susceptible necrosis reaction that develops in root tissues after they are infected and colonized by P. aphanidermatum and other Pythium spp. Browning is associated with accumulation of phenolic polymers, which in part may become bound to cell walls of root tissues (20, 72). In recent studies, concentrations of bound phenolics, which include simple as well as polymerized forms, greatly increased in pepper roots inoculated with P. aphanidermatum, but remained low in noninoculated controls (72, 103). Concentrations of free phenolics, however, increased only slightly in inoculated roots and were similar to those in noninoculated roots. While it is possible that synthesis of free phenolics did not increase in inoculated roots, it is more likely that synthesis did increase but that concentrations in the roots remained low because of systemic transport to other parts of the host or release into the nutrient solution, as was found in hydroponic lettuce (11). Most phenolics in higher plants are derived at least in part from phenylalanine, a product of the shikimic acid pathway. Deamination (release of ammonia) from phenylalanine by phenylalanine ammonia lyase (PAL) yields trans-cinnamic acid, derivatives of which are simple phenolic compounds called phenylpropanoids, important building blocks of more complex phenolic compounds (105). Elicitors of P. aphanidermatum and other pathogens are known to increase PAL in cultured plant cells and protoplasts (91). Collectively, the available evidence indicates that P. aphanidermatum and other Pythium spp. markedly activate the shikimic acid and phenylpropanoid pathways, and promote biosynthesis of phenolic compounds in infected roots.

Recent findings point to the exciting possibility that Pythium aphanidermatum triggers necrosis in host roots by means of a specific elicitor. Veit et al. (113) sequenced a secondary metabolite of the pathogen, and determined its ability to induce cell death in carrot, Arabidopsis, and tobacco. The metabolite, called the P. aphanidermatum necrosis-inducing elicitor or PaNie, has a high degree of sequence similarity with necrosis-inducing elicitors of Phytophthora spp. and Fusarium oxysporum (3, 24, 81). Evidence indicates that PaNie induces the phenylpropanoid pathway in the host (91).

Symptoms on aerial portions of crops affected by Pythium root rot often include stunted shoots, wilted leaves, and smaller, fewer fruits (71, 122). The leaf canopy of sweet pepper, cucumber, and several other hosts normally remains green until root rot is extremely severe such that many roots are brown, decayed and fragmenting. Moreover, in our experience, leaves of pepper plants inoculated with P. aphanidermatum or P. dissotocum within a few days often appear darker green than those of noninoculated control plants. Foliage of commercial crops with severe root rot often appears in good health except that it is typically stunted. Canopy stunting may go unnoticed for a considerable time because all plants in the cohort are similarly affected (71, 98). Pythium root rot stunts shoot growth and reduces flowering and fruiting long before the foliage wilts or becomes chlorotic. Wilting is initially temporary, often in association with high daytime temperatures, but in many instances becomes permanent. Under some conditions, severely-affected peppers and certain other kinds of hydroponic crops are able to regenerate roots sufficiently to sustain green foliage for long periods, but growth and productivity normally are reduced or poor.

Inoculum production

Pythium aphanidermatum, P. dissotocum and Pythium group F produce abundant sporangia, zoospores, and oospores in association with roots of hydroponic crops. Sporangia are generally formed on mycelium at or near the root surface (86). Sporangia of P. aphanidermatum have been encountered also in association with mycelia in cucumber root exudates floating in the plant nutrient solution (122). In field soils, lobate sporangia such as those of P. aphanidermatum may survive only a day or two, and are exogenously dormant on account of microbiostasis (61, 96, 97). The survival of sporangia and their ability to produce zoospores in root zones of hydroponic crops is not well-understood. However, zoospores can be found in nutrient solutions during most phases of root rot epidemics, though are sometimes difficult to detect (53, 66, 71, 100). The majority of zoospores probably arise from sporangia on mycelium while some may form from sporangia formed from germinated oospores (97). As in all Pythium spp., the zoospores are not formed in the sporangium itself but in a vesicle outside it (112).

Oospores of P. aphanidermatum, P. dissotocum, and Pythium group F form in infected roots, especially in cortical tissues, and can be found on mycelia in nutrient solution surrounding the roots and rooting media, such as among slivers of rockwool. In investigations of hydroponic peppers inoculated with P. aphanidermatum or P. dissotocum, oospores were less numerous in discoloured (brown) portions of roots than in portions that were not discoloured (73). Density of oospores in the roots varied markedly with pathogen isolate. Oospores are the principal survival structures of Pythium spp. (61), but quantitative aspects of oospore survival in hydroponic systems remain to be explored. Based on observations of P. aphanidermatum and other Pythium species in soils and host residues (61), oospores may survive for at least several months in fragments of dead roots or in a free state in troughs, used rooting media, and plumbing components of hydroponic systems. Oospores exhibit constitutive dormancy, so that some of them do not germinate even under conditions conducive to the germination of mature oospores (61). Temperature, pH, age, and other variables influence the conversion of dormant to germinable oospores (1). Germination frequency of P. aphanidermatum oospores from culture was found to be initially low (27%) and progressively increased after 1 to 2 weeks (2), whereas only 10% of those produced in soil were capable of germination (108).

Hyphae are a further form of Pythium inoculum of potential importance in root rot epidemics. Hyphal fragments of P. aphanidermatum and P. dissotocum recovered from nutrient solution in root zones of peppers grown in small-scale trough systems were, in most instances, associated with fragments of rotted roots (A. Khan, N. Owen-Going & J.C. Sutton, unpublished). However, dissociated hyphae are easily overlooked. Growth of hyphae of P. aphanidermatum from slimy masses of root exudates in the nutrient solution to roots of cucumbers was reported in trough systems (122). The role of hyphae in plant to plant spread of P. aphanidermatum was demonstrated in cucumbers grown in rockwool slabs with recirculating nutrient solution amended with a nonionic surfactant to inactivate zoospores (100).

Inoculum dispersal

Circulating nutrient solution is a principal means by which propagules of Pythium are dispersed in hydroponic crops. Patterns and rates of dispersal of Pythium propagules can be expected to vary in relation to the type of propagule, pattern and dynamics of flow of the nutrient solution, and impediments in flow paths such as roots, rooting media, and physical components and structure of the hydroponic system. Motion of nutrient solution through root zones in hydroponic troughs with no rooting medium (the so-called nutrient-film technique or NFT) include zones of rapid streaming between root masses and zones of slow movement or stagnation in areas occupied by roots (36). The latter increase proportionately as root systems grow. Roots, rooting media, and plumbing components in various kinds of hydroponic systems provide large surface areas on which propagules are potentially deposited or trapped. Movement of nutrient solution through collecting pipes and in mixing tanks is generally rapid and turbulent. The physical structure of the hydroponic system may markedly influence propagule dispersal. For example, when nutrient solution is fed to individual slabs of rockwool compartmentalized in plastic film and recirculated from the slabs through pipes and mixing tanks, propagules have the immediate possibility of dispersal only among the few plants, often two to six, growing in any particular slab (Fig. 1). This contrasts with troughs that each accommodate numerous, often hundreds, of plants, and which thus favor immediate downstream dispersal of propagules to many plants within the trough (Fig. 2).

Zoospores of Pythium and other Pythiaceous microbes are dispersed by transport in nutrient solution (36, 77, 99, 100) and are also motile by means of two flagella (17, 33). Zoospores of Phytophthora spp. were found to be dispersed only short distances (<10 cm) in stagnant or standing water, but comparatively long distances in flowing water (36). In hydroponic troughs in which root systems are well-developed, zoospore populations tend to be high and aggregated in zones of slow movement of nutrient solution, and zoospore dispersal is largely localized among nearby roots (36, 84). These findings, and perhaps intuition, suggest that localized dispersal of Pythium and Phytophthora zoospores may predominate in roots grown in rockwool and other media where movement of nutrient solution is slow. However, there is abundant evidence, though largely circumstantial, that portions of zoospore populations are dispersed rapidly throughout major portions of small- and commercial-scale hydroponic systems (53, 65, 66, 71, 98). When flow of the nutrient solution is turbulent, zoospores abruptly shed their flagella and encyst, thereby losing the necessary ability for chemotaxis towards potential infection sites on roots. Even minor movement and vibration of zoospore suspensions, such as in glassware on a moving laboratory cart, can trigger shedding of flagella. Observations in commercial crops of sweet pepper grown in troughs without a rooting medium indicated that few zoospores or other Pythium propagules returned to the crop in nutrient solution that was recirculated through pipes and mixing tanks (71). About 10 to 55 zoospores mL-1 nutrient solution were found at outflows of the troughs, but d"0.1 zoospores mL-1 solution were recovered, all encysted, before the nutrient solution flowed into the mixing tank, and none usually was found after the mixing tank. Zoospores probably were not recovered on account of deflagellation, lysis, encystment, sedimentation, adhesion to various surfaces, inactivation, and death.

Dispersal of oospores and hyphal fragments of Pythium in hydroponic nutrient solutions has been reported only incidentally (39, 71, 100). It can be anticipated that these propagules are dispersed as free entities or in association with root fragments, especially during stages of epidemics when roots are rotting. Fragments of sloughed root cortices with oospores and hyphae of P. aphanidermatum were frequently observed in epidemics of root rot in small-scale trough systems (J.C. Sutton, A. Khan & N. Owen-Going, 2002; unpublished). In contrast to oospores, hyphal fragments of Pythium spp. are short-lived in many environments (61), yet there remains the possibility that they may survive long enough to make contact and possibly infect other roots. Based on apparent circumstantial evidence from cucumbers in small-scale hydroponic systems, Stanghellini et al. (100) concluded that hyphal fragments either do not occur or do not function as effective inoculum for dissemination in recirculating nutrient solution.

Pythium propagules may be dispersed between troughs, or among slabs of rockwool or other rooting media by fungus gnats and shore flies (discussed above), splashing water, greenhouse equipment, and workers (29, 39, 40, 59). Water dripping from the greenhouse roof can often splash onto exposed rooting media, nutrient solutions, plants, and the greenhouse floor, thus affording the possibility of splash dispersal of Pythium propagules. Transmission of only a few propagules from trough to trough or slab to slab may allow Pythium to establish effectively in previously uncontaminated root zones (66). Several investigators have reported trough to trough transmission (66, 98, 100, 116), though the mode of transmission was usually not known. While dispersed zoospores may encyst and so become immobilized, the cysts are more resistant to extremes of temperature, desiccation, or ionic environment than are zoospores (33). However, their ability to adhere to roots and other surfaces decreases over time.

Epidemiology of root rot

An understanding of root rot epidemics is fundamental to the development and refinement of methods and practices to manage the disease in hydroponic crops, but remains fragmentary. While root rot is almost universal in commercial hydroponic systems, and in many instances becomes sufficiently severe to cause serious crop losses, it is also true that in many other instances progress and spread of the disease is comparatively slow and losses are perceived as minor. From the literature, and an abundance of anecdotal evidence from greenhouse growers and crop advisory personnel, it is clear that a myriad of variables significantly influence Pythium species and root rot development in hydroponic systems. Important variables range from subtleties such as calcium in the root zone, which at millimolar levels strongly influence adhesion of zoospores to roots (33), to elevated temperature, which can bring about abrupt and explosive increases in root necrosis in large portions of crops (4, 28, 39, 54, 61, 83, 107). This portion of the present article will now focus on effects of factors associated with the host, the pathogen, the environment of the nutrient solution and plant canopy, and human interferences on disease severity and pathogen ecology. Excellent perspectives of the general principles of epidemiology are given in Zadoks & Schein (119) and Bergamin Filho & Amorim (7). For a critical review of the epidemiology of diseases caused by Pythium spp. in plants grown in soil, attention is drawn to Martin & Loper (61).

Host Factors

To our knowledge, all cultivars of all vegetables and flowers produced in greenhouse hydroponic systems in Canada, the USA, Brazil, France, and other countries are susceptible to moderate or severe epidemics of Pythium root rot. These crops include cucumbers, tomatoes, sweet peppers, lettuce, spinach, arugula, nasturtium, and roses. Other crops such as chrysanthemum and antirrhinum have not yet been widely successful in hydroponic systems in part on account of severe root rot. In Canada, casual observations and anecdotal reports indicate that tomatoes generally are less severely affected than are cucumbers and sweet peppers. We are not aware of any published reports of quantitative differences in cultivar susceptibility to root rot in various hydroponic crops, but in some instances growers consider that some cultivars of hydroponic vegetable crops are less susceptible than others. Kamoun et al. (52) reported differences in host susceptibility to Pythium root rot based on plant age and tissue development.

Susceptibility to Pythium root rot is normally reported in terms of the severity of browning (or other discoloration) and fragmentation of the roots, or as root growth parameters such as volume, length, fresh mass and dry mass. Estimation of symptoms, however, focuses only on the necrotrophic phase of disease and neglects susceptibility to the biotrophic phase including the infection process and colonization. In our experience, root systems of peppers and chrysanthemums inoculated with P. aphanidermatum or P. dissotocum in small-scale hydroponic units under some conditions were symptomless yet heavily colonized by the pathogen. Other investigators reported similar findings with P. dissotocum and Pythium group F (86, 98). Further, we have frequently noted that a large proportion of colonized but symptomless roots (e.g. 30-70%) turn brown during a short interval (e.g. 12 to 24 h) between observations. Critical assessment of susceptibility requires estimation of infection frequency and colonization in inoculum dose-response studies, as well as estimation of symptoms. Environmental conditions need to be critically controlled in view of the sensitivity of colonized roots to the transition from biotrophy to necrotrophy.

Phenological growth stage and age of roots influence root rot severity in hydroponic crops but quantitative relationships of these variables and rates of disease increase are not well-understood. Root browning and rotting often progress for almost the entire life of the crop, which in Canada is about 4 months for cucumbers, 10 to 11 months for tomatoes, and 10 to 18 months for sweet peppers. Each of these hosts has an extraordinary ability to continually produce new roots, which frequently become attacked by Pythium. In general, zoospores of P. aphanidermatum, P. dissotocum, and Pythium group F, as well as other Pythium species, infect young roots regardless of the phenological growth stage of the host, but do not infect older roots (33, 61, 89). However, Zheng et al. (122) found that hyphae of P. aphanidermatum growing from root mucilage in the nutrient solution invaded young and old cucumber roots. In this study, root growth, quantities of root mucilage, and percent discolored roots oscillated in patterns that were similar and synchronous, which suggested that the dynamics of root growth and mucilage accumulation are fundamental factors contributing to patterns of root browning and rot associated with P. aphanidermatum. Root mucilage is readily utilized by rhizosphere bacteria (55), including microbial agents that, by destroying the mucilage, may restrict saprophytic development of P. aphanidermatum and its ability to attack roots via hyphae (122). In general, qualitative and quantitative shifts in mucilage and other exudates from roots at various stages of crop development appear to markedly affect root zone microbes including Pythium spp., which depend on exogenous nutrients for germination and infection (61).

Recent studies in our laboratory have demonstrated for the first time that environmental stress factors can increase the susceptibility of hydroponically-grown plants to Pythium root rot. The studies were conducted using pepper plants in single-plant hydroponic units with aerated nutrient solution. When temperature of the nutrient solution was raised to 28ºC or 34ºC for a few hours or days and subsequently lowered to 22-24ºC prior to inoculation of the root systems with P. aphanidermatum, root browning progressed earlier and more rapidly than in control plants maintained at 22-24ºC before inoculation (C.R. Sopher & J.C. Sutton, unpublished). Similarly, when plants were kept in nutrient solution (pH 5.8) amended with certain simple phenolic compounds for 48 h and then placed in unamended solution for 24 h before inoculation with P. aphanidermatum, disease severity increased more rapidly than in untreated controls (103). This finding suggested that phenolics escaping from diseased roots might predispose downstream healthy plants to attack by Pythium. Collectively, the studies demonstrated that high temperature and phenolic compounds predisposed the plants to root rot. By definition, predisposition refers to increased susceptibility of plants to disease brought about by environmental factors acting prior to infection by the pathogen (39). It is likely that other environmental stressors or stress conditions, such as low intensity light and low concentration of dissolved oxygen in the nutrient solution also predispose plants to Pythium root rot. Chérif et al. (14) found that Pythium F colonized roots of hydroponically-grown tomatoes more extensively when concentration of dissolved oxygen was moderate (5.8-7.0%) or low (0.8-1.5%), than at high levels (11-14%), however, it was not determined whether the influence of reduced oxygen was through increasing susceptibility of the host, or through effects on the pathogen or the host-pathogen interaction.

Pathogen Factors

Substantial intraspecific variation in pathogenicity and virulence exists in species of Pythium that attack hydroponic crops. Symptoms produced by isolates of P. dissotocum in pepper varied widely but generally overlapped with those caused by other isolates, and included zones of root-tip browning of different sizes and hues, different severity of expansive root yellowing, architectural changes, root swelling, and callus cell proliferation (73). Isolates of P. aphanidermatum usually are highly virulent in hydroponic crops, but variation in virulence was reported in tomato (32) and among twelve plant species grown in soil (63). Rafin & Tirilly (82) reported that isolates of Pythium group F variously caused localized necrosis at root apices and more severe and progressive root rot. In view of the intraspecific variation in P. aphanidermatum and P. dissotocum, the variation in Pythium spp. grouped as Pythium F, and the environmental sensitivity of symptom expression by various Pythium strains, it is probable that the composition of Pythium species and strains in a given crop can markedly influence patterns of root rot epidemics (71, 73).

Environmental and Microbial Factors

Development of severe root browning and root rot in hydroponic crops produced in greenhouses in Canada often coincides with hot weather when temperature of the nutrient solutions and of the greenhouse in general is high. Some growers have replenished the nutrient solution with fresh solution prepared with cool water from wells to help alleviate the problem. Pythium aphanidermatum is widely known to cause severe symptoms of root rot in various crops when root zone temperature is moderate or high (e"23-27ºC) (4, 28, 61, 73, 107). In chrysanthemums grown in single-plant containers with controlled root-zone temperature, the pathogen caused progressively more severe root rot symptoms with increase in temperature from 20ºC to 32ºC (Fig. 3). In a parallel study, progress curves for root discoloration caused by P. dissotocum were similar at 24-32ºC, but lower at 20ºC. Root disease caused by P. dissotocum in spinach was reported to be severe at 21-27ºC but even more severe in winter months when nutrient solution temperatures were low (28). Fortnum et al. (26) found that root necrosis caused by P. myriotylum in tobacco seedlings in a greenhouse float system was lowest when the nutrient solution temperature was 15ºC and highest at 30ºC. It is important to recognize that effects of temperature on symptom development can differ markedly from those when the pathogen colonizes the tissues symptomlessly during the biotrophic phase. In our experience, roots of hydroponic peppers and chrysanthemums can be extensively colonized by P. aphanidermatum or P. dissotocum but remain almost symptomless at 16 to 18ºC, yet develop severe symptoms within minutes or hours when the temperature is raised to 24-28ºC (N. Owen-Going, W. Liu & J.C. Sutton, unpublished). Temperature also differentially affects other stages of Pythium infection cycles such as the production, dispersal and germination of zoospores, oospore germination, and infection processes (61). The progress curves of root browning represent integrated effects, both direct and indirect, of temperature on the pathogens and their interactions with the roots.

Concentration of dissolved oxygen in the nutrient solution is a critical factor influencing root rot and crop productivity (14, 38, 88, 120). In general, root rot increases when oxygen levels are low (14). Gases move to and from roots of plants in many types of hydroponic systems chiefly by mass flow of gas dissolved in moving solution, which contrasts with diffusion through gas-filled pores as occurs in soils. Oxygen concentration in the root zone of hydroponic crops is commonly 6 to 8% (123) and growers have been encouraged to maintain a minimum of 5 mg oxygen L-1 nutrient solution (36). Concentration of dissolved oxygen can quickly decline, however, especially when temperature of the nutrient solution is high. In the absence of biological factors, the level of dissolved oxygen in water declines, for example, from about 9 to 7 mgL-1 as temperature increases from 20 to 35ºC at 101.3 kPa and 100% relative humidity. Of greater importance, however, is greatly increased demand for oxygen by roots and root-zone microbes as temperature increases, factors that become particularly important when crops have produced dense masses of roots and when microbial populations are high. It has been further estimated that a crop that is environmentally stressed requires about ten times more oxygen than one not under stress (39, 94). While allowing the nutrient solution to free fall back into the nutrient recharge tank helps to maintain adequate oxygen levels, injection of oxygen directly into the solution may be needed, especially in continuously recirculating systems. Oxygenation is one of the few practical measures available to growers when root rot is well advanced, and helps to avoid further necrosis, disintegration, and sliminess of the roots (39). The observations of Chérif et al. (14) suggest that elevated levels (e.g. 11-14%) of oxygen would be advantageous in protecting roots and promoting crop productivity.

Recirculation of nutrient solution in greenhouse hydroponic systems increases the risk of accumulation of phenolic and other organic acids to phytotoxic levels in the root zone (49, 50, 51, 114). These organic compounds are excreted as root exudates and by rhizosphere microbes, and are also released by constituent devices in the growing system (114, 117). Concentrations of total phenolic compounds of 23-30 µg gallic acid equivalents per litre of nutrient solution were found in root zones of 6-month-old hydroponic pepper crops in Ontario (T.N. Owen-Going and J.C. Sutton, 2004, unpublished observations). Phenolic compounds commonly associated with roots and nutrient solution of tomatoes and peppers, for example, include benzoic, caffeic, chlorogenic, ferulic, p-hydroxybenzoic, salicylic, and vanillic acids, most of which produce phytotoxic effects at 200 to 400 µM in the nutrient solution (10, 49, 72). Rhizosphere microbes can utilize phenolic acids (8, 115), and the rate of utilization is substantially affected by oxygen concentration (74). Thus, microbes can potentially ameliorate the toxicity of phenolic compounds to hydroponic crops (11). Recent studies of hydroponic peppers in our laboratory demonstrated that several phenolic acids applied in the nutrient solution at a final concentration of 200 µM exhibited allelopathic effects, but concentrations of 2 to 200 µM also predisposed the plants to root rot caused by P. aphanidermatum (72, 103). In this system allelopathic phenolics rapidly increased in roots infected by P. aphanidermatum, promoted sporangia production by the pathogen, leaked into the nutrient solution, exhibited toxicity to pepper, and predisposed pepper to attack by the pathogen. Based on these findings, it was hypothesized that P. aphanidermatum, by increasing phenolics in roots, initiates autocatalytic cycles of events that accelerate root rot epidemics and health decline in peppers (103).

Hydroponic systems are often extraordinarily conducive to root rot epidemics in part because the root zones lack communities of microbes that can effectively antagonize pathogenic species of Pythium associated with the roots, rooting media, and nutrient solution. In contrast to microbial communities in natural soils, microbial diversity and density in hydroponic systems are frequently low. A majority of hydroponic crops are germinated (or otherwise propagated), and subsequently transplanted, in hydroponic units with rooting media, nutrient solution, and components such as plastic containers, troughs, and tubes that contain comparatively few microbial propagules. It can be anticipated that, during crop development, further incidental microbes enter the root zone, and that the density and diversity of microbial communities increases as availability and diversity of food sources from rhizodeposition increase, but published data to support this are sparse. In cucumbers transplanted into small-scale hydroponic trough units with recirculating nutrient solution, estimated density of fungal propagules was initially about 102 colony forming units (cfu)/mL, increased to near 104 cfu/mL by day 53, and remained near this value until the study ended on day 102 (122). Bacterial density increased from slightly below 105 cfu/mL to peaks of 108 - 109 cfu/mL at 74 and 95 days. Propagule density of Pythium, Penicillium, Fusarium, other fungi, and bacteria was, in most cases, one hundred to one thousand times greater in the root mucilage than in adjacent nutrient solution. Patterns of increase in density of bacterial and fungal propagules in the nutrient solution were also reported for rockwool-grown tomatoes at about 4 to 6 months after transplanting (109).

While buildup of root-zone microflora during the life of a hydroponic crop can be substantial, sanitation practices and use of new plastic materials and rooting media preclude all but minor carry-over of microflora into the subsequent crop. Thus, the very practices that aim to eliminate pathogens also remove microbes that potentially antagonize pathogens, which again contrast with crops grown in natural soils. However, some hydroponic cucumber growers in Ontario, Canada, boldly reused rockwool slabs for up to four successive crops and found that root rot was always less severe in the used slabs than in crops where new slabs were used. These anecdotal reports were consistent with recent scientific findings that the microflora of used rockwool plays an important role in suppressing root and crown rot symptoms caused by P. aphanidermatum in cucumber (79). These investigators also found that suppressiveness was easily transferable between water-saturated rockwool slabs, and was associated with differences in structure of bacterial populations as visualized by using polymerase chain reaction (PCR) followed by denaturing gradient gel electrophoresis (DGGE). Despite the suppressiveness of used rockwool, it would be inappropriate to advise growers to reuse rockwool slabs on account of the potential presence of other pathogens and pests. Subsequent investigations of the rhizosphere microflora in cucumbers grown in used rockwool revealed that fast-growing bacteria predominated at the root tips, whereas slow growing bacteria were most abundant at root bases (25). Further, the proportion of fast-growing bacteria decreased as plants developed through vegetative and reproductive stages, even on root tips, which are young tissues regardless of plant phenological growth stage. DNA microarray technology, combined with PCR-DGGE and conventional colony counts on agar media, should allow critical characterization, profiling, and tracking of root zone microflora and their relationships to pathogen suppression.

General experience in the greenhouse industry indicates that the type and structure of hydroponic systems greatly influence severity of root rot epidemics in hydroponic crops. In Canada, reports of severe epidemics are more frequent for crops grown in trough systems, in which the nutrient solution circulates among roots of scores, hundreds, or thousands of plants before returning to the mixing tank, than in highly compartmentalized systems such as rockwool slabs in which only a few plants share a common root zone. The industry findings are consistent with expected patterns of pathogen dispersal, especially zoospores, in relation to compartmentalization of root zones. Risk of severe root rot is also considered higher in systems with continuously recirculating nutrient solution ("closed systems") compared to those where the solutions are allowed to run to waste ("open systems") (42, 64). However, in a study of tomatoes grown in rockwool, incidence of colonization of roots by Pythium was higher in an open system than in a closed system (109). In container-grown crops, which generally are ornamentals such as gerberas and roses, root rot is normally more severe when the containers are positioned on the bottom of wide-based troughs through which nutrient solution is allowed to flow, as opposed to when the containers are elevated well above the troughs and the plants are fed entirely through capillary tubes positioned in the rooting medium. Effects of the presence and type of rooting medium on root rot epidemics in commercial hydroponic systems are not well-understood. In Ontario, Canada, root rot epidemics in pepper and tomato have tended to be severe when crops were grown in trough systems with rockwool or with no rooting medium. Some growers reported fewer root rot problems when they used coconut fiber or certain grades of peat as opposed to rockwool. When containers are used in hydroponic systems it would probably be advantageous to use pathogen-suppressive rooting media (34, 35).

Human Interferences

Numerous practices used to produce and protect hydroponic crops influence the incidence and patterns of increase and spread of root rot epidemics. It is outside the scope of this article to review what is known of these practices in relation to root rot, however principal measures that are used, or that have potential use against root rot in various kinds of hydroponic systems are summarized in Table 1. Further details are available in the following publications: Bélanger & Menzies (5); Bélanger et al. (6); Chatterton et al. (12); Chérif & Bélanger (13); Ehret et al. (18); Evans (22); Folman et al. (25); Jarvis (39); Jensen & Collins (42); Jung (49); Khan et al. (53); Lopes (59); Lopes et al. (60); Menzies & Bélanger (65); Paulitz (75); Paulitz & Bélanger (76); Punja & Yip (80); Runia (90); Schuerger (93); Sutton et al. (104); Utkhede et al. (110); Zheng et al. (122)

From the epidemiologic perspective, disease management practices can achieve two principal effects (7, 119). First, they can reduce the level of initial (or primary) inoculum of the pathogen in the crop environment. This is the inoculum that can initiate the epidemic, analogous to a match lighting a fire. Secondly, they can reduce the rate of increase in severity of the epidemic, analogous to the rate at which the fire burns and spreads. In Table 1, practices that are thought to influence chiefly the initial inoculum of Pythium are distinguished from those that help to keep down the rate of increase of root rot after epidemics have begun (eg 19, 30). In Ontario, infected transplants, though often symptomless, frequently contribute to the initiation of root rot epidemics in crops. Thus root rot control should begin with the seed, cutting, and other propagative material.

Among the practices that aim to reduce rates of increase of root rot, it is important to distinguish the epidemic impact made by disinfesting the nutrient solution as it recirculates outside of the crop from that made by treatments that suppress Pythium in the roots and root zone of the crop (Table 1). In tomatoes (121) and in our investigations with lettuce and chrysanthemums in small-scale trough systems (18-20 plants per trough) with recirculating nutrient solution (44, 71) treatment of the solution with ultraviolet radiation (UV-C) at doses sufficient to kill Pythium propagules gave little or no suppression of root rot. Similar treatment in a commercial-scale pepper crop in hydroponic troughs (NFT) did not significantly suppress root rot increase (102). These findings are not surprising given that most Pythium zoospores are dispersed locally among roots in the crop, and of those in effluent from troughs few survive the turbulent ride in nutrient solution through pipes and mixing tanks to the UV apparatus. Emphasis is needed on treating the root zone, such as with microbial agents, to protect the roots.

Several strains of specific microbes have been identified that have strong potential for controlling root rot in various kinds of hydroponic crops. They include Pseudomonas chlororaphis Tx-1 (= Pseudomonas aureofaciens Tx-1) (12, 53). Pseudomonas fluorescens 63-28 (58, 76) Comamonas acidovorans C-4-7-28 (58), Bacillus cereus HYU06 (58), Bacillus subtilis BACT-O (111), Gliocladium catenulatum J1446 (80), Lysobacter enzymogenes 3.1T8 (25), and Clonostachys rosea (J.C. Sutton, 2003, unpublished observations). In our experience in commercial systems, biological control treatments should begin when plants are at the seedling or rooted-cutting stage, though good control is often possible in older plants in which disease has already begun to increase. Certain agents, such as P. chlororaphis, in some instances protect crops for several weeks or months without need for reapplication. Several of the microbes also appear to induce systemic resistance to powdery mildew and other foliage diseases (example: 122).

Symptom development, growth of the shoots and crop productivity

A surprising aspect of hydroponic crops with Pythium root rot is that the foliage often appears green and healthy even when root rot has become severe. Foliar discoloration normally develops only when the root systems have become almost entirely rotted. Under controlled conditions, the leaf canopy of pepper plants inoculated with P. aphanidermatum or P. dissotocum often becomes darker green than that of the noninoculated controls, which may indicate an important role of growth regulators in development of foliage symptoms.

The relationship of root disease caused by the various Pythium species and plant growth is currently understood chiefly from investigations in small-scale hydroponic systems. Reduced mass of roots and shoots, and reduced yield and quality of fruits or flowers were noted for several crops (12, 45, 53, 54, 65, 66, 71, 110). In pepper, P. aphanidermatum reduced the volume, fresh and dry mass, total length, and surface area of the roots, as well as total leaf area, and height, fresh mass and dry mass of the shoots over two to three weeks following inoculation (53, 72). In other experiments, concentrations of chlorophyll a, chlorophyll b, and total carotenoids expressed based on leaf area or fresh mass, were significantly higher in inoculated plants than in noninoculated controls (C.R. Sopher, 2003, unpublished).

The first characterization of alterations in whole-plant photosynthetic rate and carbon assimilation associated with Pythium infection of the roots was recently described in hydroponic peppers (45, 46, 47). Inoculation of plants with P. aphanidermatum resulted in reduced whole-plant net carbon exchange rates (NCER), and a loss of 28% in cumulative carbon gain within 7 days. Leaf area, and dry weight of the shoots and roots, were significantly decreased, and the shoot:root ratio was higher in inoculated than in noninoculated plants. However, no differences were observed in NCER and evapotranspiration in inoculated compared to control plants when data were expressed based on leaf area and root dry mass, respectively. Thus Pythium infection did not appear to affect the photosynthetic apparatus directly, and the reductions in photosynthesis and growth were not caused by inefficient water transport by diseased roots. The main effect of root rot was to suppress the rate of increase in leaf area of the plants, as opposed to influencing the efficiency of photosynthesis per unit leaf area.

Implications and possibilities for root rot management

The knowledge and understanding of the etiology and epidemiology of Pythium root rot provides a valuable platform for rationalizing new research directions and developing better technologies and practices for managing root rot in hydroponic crops. Epidemiological information suggests that focus is needed in direct protection of roots against Pythium through treatments applied in the root zone, and that protection is needed beginning at the seedling stage and throughout a major portion of the crop cycle. Collectively, available data suggest that treatments that kill or inactivate Pythium in nutrient solution as it recirculates outside the crop are at best marginally effective in reducing the progress of root rot in large- and small-scale hydroponic systems. Such treatments may be important, however, for reducing the introduction of Pythium into hydroponic crops, and for destroying other pathogens, including viruses and bacteria.

Technologies to facilitate tracking of Pythium spp. and root disease are an obvious step in optimizing effectiveness of root-zone treatments such as use of microbial agents and oxygenation of the nutrient solution, as well as other measures to control root rot. Roots of hydroponic crops are generally out of sight and not easy to examine with any thoroughness, so that severity of root browning is difficult to determine. Further, visual examination does not detect infected roots that happen to be symptomless. Antibody-based dipsticks, other immunoassay-based diagnostic kits, and DNA microarrays that allow tracking of Pythium have already been developed (57, 62, 77). Such assays have potential applications in standardizing the health of propagative materials, and to minimize the risk of introducing Pythium into hydroponic systems in, for example, the roots of transplants growing in rockwool cubes. They can also be used to detect or roughly quantify Pythium throughout later stages of crop development. Detection of Pythium does not necessarily imply that there is, or will be, a destructive epidemic of root rot. As summarized in previous sections of this article, development of severe root rot depends on numerous environmental and host factors, particularly high temperature and reduced levels of dissolved oxygen in the nutrient solution. A much better quantitative understanding of environmental variables in relation to root rot, and especially environmental stressors that predispose roots to Pythium attack, would open the doors for root rot prediction, and alerts for remedial action, driven by sensors in the canopy and nutrient solution. After decades of speculation, it is time to put some numbers on environmental stress conditions in relation to root rot. The recent findings that phenolic compounds accumulate in the nutrient solution, especially during root rot epidemics, and can threaten crop health directly and by predisposing plants to Pythium attack, signify a need for further investigations of phenolics, including any necessity for remediation of the solutions against phenolics. The new understanding of physiological responses of the plant canopy to root infection by Pythium (46) has paved the way for determining root disease severity by remote sensing in the canopy, and thereby avoiding the frustrations of examining roots directly.

Introduction of microbial agents, manipulation of the root zone microflora, oxygenation of the nutrient solution, and regulation of nutrient solution temperature are among the best available approaches to suppressing Pythium in the root zone, but each requires considerable investigation to provide practical and dependable know-how for growers. Much headway has been made in assessing effectiveness of microbes against Pythium in short-term experiments, but critical knowledge needed for long-term root protection throughout crop cycles, and in the face of shifts in the general microflora, chemical environment, and physical conditions in the root zone, is relatively sparse. Better understanding of the root-zone microflora in relation to Pythium, root rot, plant growth and disease resistance, root mucilages, allelopathic compounds of plant or microbial origin, and other key variables, should be possible with the aid of DNA microarray and other recent technologies to detect major microbial species and genes in the hydroponic system. Data banks of dissolved oxygen levels and temperature of the nutrient solution in relation to important variables such as Pythium, microbial agents, other microflora, root rot, and crop growth and productivity are needed to develop protocols for their rational use and to adequately understand the value of such use. Given the inadequate levels of host resistance to Pythium, new approaches to improve resistance such as through antibody-based mechanisms justify vigorous exploration. Fundamentally, new levels of integration of management practices against Pythium root rot and other diseases are needed that are appropriate to the kind of hydroponic system, whether sophisticated as in many greenhouses in Canada, the USA and some European countries, or simple yet functional like many of those in Brazil and other countries with warm climates.


The above information was found here.


An ounce of PREVENTION is worth a ton of CURE.

Keep the area, especially the tank and water neat and clean. Keep the dead leaves out of the water.

Thick, fat, white furry roots are what you most want to see - they are absolutely indicative of healthy root growth. Be aware however that the color of a nutrient solution will stain the roots, turning them yellow or brown. This is also true of many nutrient additives. Older, more mature plants will have a darker cream-colored root system, and some plants just tend to have a natural cream colored color pigment.

I like my roots to smell like fresh cut lettuce or like fresh alfalfa srouts.

Young healthy roots:




Mature Roots:



Root Zone Temperature
The temperature of the root zone and the temperature of the nutrient water solution can have a major effect on the healthy growth and appearance of the root system. In general the temperature should be between 68 and 72 degrees farenheit. Anywhee between 64 and 80 will be OK. Colder or warmer conditions can cause poor and stunted root growth, as the roots don't want to grow into the unhospitable nutrient solution. Major root death can occur in even brief periods of extreme heat stress. Poor temperature conditions leave the door open to root disease.

Some would argue that one of the strengths of hydroponics is its sterile environment, and the notion of exposing growing systems to bacterial and fungal organisms would be self-defeating, if not sacrilegious. These growers rely on sterile growing environments, strong disinfectants and a product like SM-90. Another option is Hydrogen Peroxide. Each of these offer their own protection and benefits. But NEITHER SM-90 or Hydrogen Peroxide works well with organic nutes or organic additives in the reservoir. You can not try to kill the organic algae or pythium and add organic materials back. That just won't work!
They do not work well together and SM-90 has also been known to react poorly with Superthrive.
In a sterile growing environment, your goal is to have a super clean reservoir. This is harder than it sounds. Folks who have been growing in the same area with the same equipment for years might find that they are suddenly having root problems when they never had them before. Or a new grower might begin having problems right from the beginning because of his water or temps.
Keeping your reservoir totally sterile can work very well, but once you get a population of icky badness it will keep coming back again and again. Some pathogens such as pythium are almost impossible to get rid of completelely. No matter how many times you sterilize everything with a bleach solution, the problem returns. It can get very frustrating and expensive to constanly be battling. More and more innovative growers are moving toward a more wholistic approach of using good microbes in the reservoir with organic nutes or staying with strickly chemical nutes..
One option is to use no organic additives at all and to rely strictly on chemical nutrients based on fertilizer salts. We think a better choice is to continue using organic material, but also using an enzymatic addtive like Hygrozyme that will break down the unwanted organic matter in the reservoir . If you would like to use additives such as bat guano, compost or fish-based products, you might consider run to waste instead of a recirculating system.
AGAIN,
Hydrogen Peroxide DOES NOT work well with organic nutes or organic additives in the reservoir. You can not try to kill the organic algae or pythium and add organic materials back TO THE WATER. That just won't work!

The above information was found here

Below is info about a product that kills root rot. I haven't used it so make your own judgement.

Prevention is certainly better than cure. Every effort should be directed toward preventing the disease before it begins. Prevention begins with a policy of total cleanliness. Hygiene is a primal requirement for overall disease control. Following is a list of tips that should be implemented.

• The entire growing area should be kept clean at all times.
• Dead leaves should always be removed from the plant and destroyed.
• Keep all soils and composts out of the grow-room or greenhouse.
• Do not convert plants from soil into hydroponic systems.
• Do not enter the growing area with outdoor footwear on.
• Do not bring any plant material in from outside if you can avoid it.
• Sterilise all pots, gullies, trays and tanks between crops.
• Nutrient tanks should be kept scrupulously clean.
• Do not allow soil or plant material to fall into tanks at any time.
• Keep tanks covered except for airflow requirements.
• Disinfect all surfaces, tools and equipment that will contact the plants or the nutrient solution.

Top of page

System Sterilization

Along with good hygiene practice, a practical method to keep Root Rot under control in most circumstances is to add a sterilising agent every week to your nutrient solution. This has the capacity to eliminate all pathogenic organisms from the nutrient tank. This is a preventative method and should eliminate Root Rot before it can take hold. However, you must still be vigilant as your plants may still get Root Rot if a large outbreak is introduced to your system. This will be too much for your sterilising agent to handle.

Calcium Hypochlorite can be used to sterilise systems and is a very affective sterilant. Calcium Hypochlorite however does not easily break down and a residual remains in the system that can be very damaging to new plants.
There are now new products on the market that are designed specifically to sterilise plant and equipment in horticultural situations. These products destroy organisms without leaving any dangerous residues. To find these products, consult your nearest hydroponics shop.
Top of page

Treating Root Rot Outbreaks

If you have an outbreak of Pythium / Root Rot then you need introduce a chemical into your system to kill the Pythium before it kills your plants. One such chemical is our product Rebound.
Rebound kills Pythium and other pathogens in your hydroponic system. When symptoms occur, add 1 mL of Rebound per litre of water in your reservoir, and continue weekly until the symptoms clear up.
Rebound can also be used to maintain your system by adding 1 mL per litre of water into your reservoir every week.
You will need to flush your tank at least a week before harvest and not use Rebound in this period.
Rebound can also be used in run to waste systems as well as recirculating systems.
If you would like to purchase Rebound then please contact us to find your nearest hydroponics shop stocking our product. For more information see our Rebound page for product information

The above info should be ehnough to get you started in the right direction. The is a wealth of info on GC also if you take the time to look. I myself like many of you are still learning so I may or may not be able to answer your questions. Post'em and if I can I will.

Peace!!!

 

BumP

 

So far I have been fighting the dreaded rot for over a month now. I spent weeks using store bought peroxide and cleaners.

I have tried spraying the roots with a water down mix of perox and also tried trimming some of the more affected roots off. I have bleached the buckets, scrubbed them switched them and threw 1 against a well built wall. The result was, the rot would go away only to return as soon as I gave the system nutes

I then went to a hydro store and got some H2O2. Result you ask? The same!

The temp are in there mid 70's res temps high 60's to low 70's and as soon as nutes are added, BOOM! Damn shit right back!

My plants are in bad shape, the growth is stunted, leafs are buckling, bud sites show no growth.

So my advise to the grower reading this is as follows. If you catch this early then fight it hard early. Don't waste your time with store bought peroxide, (yes I know many have had great luck with store bought peroxide. Maybe they know something I don't)

Get H2O2 from hydro store. Mix it per instructions and let it run in your system for 3 days. If no signs then add a 1/4 dose of nutes with fresh water and let run 3 days. If no signs then go full dose and pray.

Clean system with diluted bleach, scrub and rinse everything air lines included. After grow don not re-use medium (hydrotron). Throw it away and get new. Spray the roots with store bought peroxide and let foam up. I have used it right out of the bottle with no ill affects. But don't use H2O2 un-diluted on the roots. Always dip the roots in a bucket of clean water to rinse them off before you put them back in the system. Make sure the water in the bucket is moving so the roots can get washed.

If you can wash the floor, walls, pumps and so on. Make sure you clean your PH tested also. Pay attention to everything!

Look for knats and bugs. I also know its cute to take pics of your pets and post online but animals and bugs is the easyist way for the rot to get into your system,

If your a com grower and if you find any 1 plant with 30% of the roots affected then in my view the growers that have told me to trash the plant and start over were correct. This problem started with 1 plant and now all have it. Had I just killed the affected plant then the others would have been fine and this grow would be over by now. Not to mention the money I have wasted trying to combat this problem.

I invite any grower here to post advise to counter or add to my advise here. I have been all over this forum and others trying to find info and the info is all over the place.

I also understand the info at the top of this post is a tuff read but 1 bad case of the rot will give you the encouragement to read the info and study. Knowledge is power.

At the time I posted the above info I tried to space it out to make it easier to read but I did something wrong cause as you can tell it didn't work. But it is readable and in truth the way you see it here is the way I found it in PDF form, Besides it's free.

The info I have given here is from personal practise in my DWC hydro system. Soil will be different. It is my hope that this info will help out someone. If you catch it early you can kill it. Just take your time don't cut corners and you may get lucky.

Peace..

 

Some of the info I posted above was on the right track but wasn't aggressive enough. I refined it a little over the last few weeks and this post is attended to be an update.

As a noob I made the mistake of thinking H2O2 was the same as store bought Perox. It isn't! H2O2 is much stronger and will eat skin and cloths. Use it always per the instructions on the bottle. The following after trial and error was my battle plan.

1. In a spray bottle, fill full of store Perox and remove plant and hold over the toliet. Spray the roots with the perox at full strength. The store bought shit isn't very strong and looses it's poentcey as soon as it it hits air. There is no danger in using it full strength. I have used it on plants as young as 2wks will no ill affects.
Also don't be easy, spray in a stream about 5in from the roots. Yes, some roots will fall off but the 1's that do are weak roots and are going to die anyway and this way you control where they land.
Let it foam then spray again. Spray until you see no snot anywhere.

Then have a clean PH adjusted bucket of water and dip the root ball in the clean water to rinse any left over crap away.

Then dump the rinse bucket, do not re-use the rinse water. Always clean rinse water per plant. Also don't cut off affected roots. The spray will knock off any roots that need removed by itself. The roots don't need your help.

2. Clean all your grow medium with bleach, IE- buckets, air lines, stones. Rinse out really well. When you think you've rinsed enough, do it again. As for the amount of bleach? It is 9 parts water to 1 part bleach. Scrub all areas with a brush. Rinse the plant from the top down with clean pure water. Just dump it and let it run from the bottom of the stem right threw the rockwool and out the bottom of the pot.

3. I use DWC so talor the followinfg to your system. Fill your system to the very bottom of the net pot with PH adjusted water. Make sure ALL the roots are in water. Leave no room for an air buffer. The reason is, the snot needs air to grow and if you cover the roots then it will look else where for a place to land. The place it will choose will be your air stones because the stone is the only supply of air.
If you only use 1 stone then added an extra 1 to your system to help collect the snot. Make sure all the stones are running in good order cause if you don't you will drowned your palnts.
Then PH the water and add 1/2 the dose of nutes. Add H2O2 per bottle and put plant back in system.

4. Now the hard part. If you want to kill the snot you MUST do this everyday for 3 days. Yea, you read that right! 3 days! New water everyday! If you do, in a week, you will kill it.

I have 3 plants in a DWC bucket and this system took me an hour and a half to do every night for a week. I did get it killed! And the knats that started it are gone to. The plants are now growing and looking better. No part of the above system hurt my plants anyway.
I invite any GC'er to post here and dispute my system or add to it. This is what has worked for me. I will also stress, if you have a comm grow then trash the plants and start over. It will take you to long to save them. If you catch it early you can kill it before it gets to bad but if more than 50% of any roots is covered and it's a comm grow, KILL it!. My system had over 80% of the roots covered in snot. This happened in 3 days with H2O2 treatment per the bottle! Yea, I caught it and used H2O2 and it still took over!
Also make sure you rinse your PH tester before you use it on a non-affected plant.

Lastly, if your brave you can take 1 cup of perlite and put it into some pantyhose. Put it in the bottom of your bucket and just leave it there. In a few weeks if your system is healthey the perlite will have little bacteria growing in it and this will help fight any little nasty that get into your system.

This shit has drove me crazy. I hope that the above info can help out someone else. I'll try to answer any questions I can, if asked. Not many GC'ers have read this thread so if you find it copy and paste the info to your hard drive. To those of you that have given me REP do to this post, I Thank You.

Good Luck.....

 

---------- bump -----------------

 

I found some more info and used this process below on 1 plant and after 2 weeks the rot was gone. I tried this because many of us don't have access to the product H2O2. So if you have a problem and you need to start treatment NOW then do the following. However don't use the following if you are using H2O2 product it will be to much for the plant. the following can be combined with the info I posted above concerning the air stones and the water level.

If you have some grungy roots on established plants, then I'd go with 1/2 cup of hydrogen peroxide (3 - 4% strength) per gallon in a full reservoir. Plan on changing the res every 4 or 5 days until the problem is under control. ​

The peroxide will kill the bacteria, and make a mess of your reservoir if the problem is progressed, so the increased res changes are important to keep things clean. There are no guarantee's when it comes to rootrot problems that are very progressed. ​

You can repeat this process until you see some new white roots developing, then keep up the 1/2 cup per gallon for the rest of the life of these particular plants.​

During this intense method, I'd drop your nutrients down to half of what you were using before until the plants show healthy new growth. ​

You should also discontinue the use of any 'live' innoculants (products that are alive like Subculture, or any mycchorizal fungus) while you're taking this approach, as the peroxide will just kill them off.​

The peroxide will kill the bad bacteria, but if the problem is too far gone, then the plants are already stressed, and the peroxide may further stress them, but we have had very positive results with this approach where nothing else will work though.

​

The 1/2 cup recommendation is half of what some of our customers run. You may need to add more to get rid of a bad problem, up to 1 cup per gallon. All of these recommendations are based on 'off the shelf' hydrogen peroxide, which is 3 - 4% strength.​

 

No 1 has posted here but me, I have had 2 PM along with rep from GC members and I thank them for doing so. But I really hoped others here would also post and maybe add there advise as well. I understand that root rot info is all over GC but that is the trouble it is all over. If members post to this thread or maybe post links to other threads with rot info then it will help out others and provide info in 1 place with the proper search tags. In the end I hope this info helps out someone and if so please post to this thread that it did.

Or maybe even post the following to this thread "Hey stupid I'm reading this thread"

At least if you post hey stupid I'll now someone is reading and getting help.

Thanks,
Peace.......

 

Thanks, Nice to know it is helping out.

Peace.....

 

Yea, foot notes. Hell I've got notes all over the place on this shit! I've even caught myself making mental notes right before I go to sleep. I think I got rot on the brain!

Thanks, for stopping in.....

 

Sorry I was offline for a few days. no, I haven't used the product above. If you do try it please post your results so others will know how it worked. The product that GC'er dsq1989 posted about I have heard from others is a good product. I haven't seen anything bad about it. Also member dsq1989 is a knowledgeable grower. I have been involved with threads in the past this member posted on and his info was dead on correct.

In short if he says it's good then trust it. The main purpose of this thread was to try and compile as much info as I could find on the rot in 1 place. But it' has become cluttered so in a few days I'm going to edit the posts and try to make them for ifo friendly.

Good luck and let us know how you kill it...

 

Unless I'm missing something I don't see a link in your post? I assume it was a link?
Please edit your post so we can read what you feel is important. We need all the help we can get with the ROT..
Peace...

 

In the above post Greendragon talked about H2O2 so I went looking for some info from another grower about use and found the following info from another site.

What causes root rot? Low Oxygen levels. (DO) Desolved Oxigen. Temp's are the main reason for root rot, Low temps will lower the DO levels and high temps will cause low DO levels. Keeping temps between 65 to 72 will allow MAX DO levels or max o2 for roots.

Over watering also causes root rot, Root Rot infects parts of the roots, which then spread infecting the whole roots causing death, Root Rot can be caused by to high of temps in hydro as well as over watering. Not enough drainage in your soil can cause Root Rot, even signs of it looking over watered, because the soil holds moisture to long for the roots to dry out. So these 3 things are all connected together. The main one that causes everything is over watering. Once you find out you have root rot, depending on how bad the problem is, you can add H202( Hydrogen Peroxide) with your water or hydro setup to kill the bacteria caused from root rot. If the plant is severely taken over by it, there are only a few things you can do, cut off the roots affected by it depending on if its hair roots or tap roots, use H202 mixed with water in a different bucket and use it for a dip to help kill off bacteria before putting it back in the system, also note the system needs to be cleaned out if you have root rot. Using products like sm90 will kill off the root rot, using h202 in your system does more harm than good, it kills off the bad bacteria and good thus creating a worse off problem after the h202 has been overran, h202 if used in a hydro/aero system must be replaced everyday due to it dissipating from the air. After it dissipates the plants defensive system has been killed off by the h202 causing the root rot that was not killed by the h202 to rebound and thus overrun the plants root system. So again h202 should never be used in the hydro system, be kept in the system, although it is safe to use to rid of algae growth in rockwool and other mediums, but never be used in with the root zones regularly. Sm90 is a good product you can use to kill off root rot. Hydroguard helps protect and there are many other products that work as well, hydroguard is one of the more popular ones that work.


To prevent Root Rot, adding product's like Thrive Alive B-1, See Weed, Super thrive will help protect your plant's from root rot and will help cure root problems.