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40°F/4.4°C? Woah thats cold! You should definitely heat the water. If you just have one bucket or two you could probably just buy one of those fish tank heaters with a thermostat and thermometer. When the lights on the temp should be around 70-77°F/21-25°C
and when the lights are off the temp should be around 60-67°F/10-15°C.
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